Abstract
A sensitive, solid phase microtitre plate enzyme immunoassay (ELISA) was developed for the determination of the herbicide metazachlor, which belongs to the class of a-chloroacetamides. The antisera were raised in rabbits by immunization with a metazachlor-BSA conjugate containing 27 metazachlor residues per molecule. A competitive ELISA with a peroxidase-metazachlor tracer was performed. Metazachlor could be detected within the range of 0.01 and 1 μg/1. The test showed no cross-reactivity with other structurally similar chloroacetamide herbicides and little cross-reactivity with derivatives of metazachlor.
| Original language | English |
|---|---|
| Pages (from-to) | 413-427 |
| Number of pages | 15 |
| Journal | Analytical Letters |
| Volume | 24 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1991 |
Keywords
- EIA
- ELISA
- enzyme immunoassay
- herbicide
- Metazachlor
- polyclonal antibodies
- solid phase