TY - JOUR
T1 - Development of an Enzyme Immunoassay for the Determination of 2,4,6-Trinitrotoluene-Probing the Influences of Humic Acids
AU - Keuchel, Claudia
AU - Weil, Ludwig
AU - Niessner, Reinhard
N1 - Publisher Copyright:
© 1992 SPIE. All rights reserved.
PY - 1992
Y1 - 1992
N2 - A new enzyme immunoassay is described which can be used for the determination of the explosive 2,4,6-trinitrotoluene in water. The assay has a detection limit of 30 ng/l. The evaluation of cross-reactivities shows that the used antibody has a high selectivity for the trinitrophenyl moiety. Since humic substances, commonly occurring in natural waters, can interact with antibodies, the obtained results can be false positive. To reduce the negative influence of the humic substances the usually performed ELISA protocol has been modified by adding a solution of bovine serum albumin (0.5% in PBS) after the coating step.
AB - A new enzyme immunoassay is described which can be used for the determination of the explosive 2,4,6-trinitrotoluene in water. The assay has a detection limit of 30 ng/l. The evaluation of cross-reactivities shows that the used antibody has a high selectivity for the trinitrophenyl moiety. Since humic substances, commonly occurring in natural waters, can interact with antibodies, the obtained results can be false positive. To reduce the negative influence of the humic substances the usually performed ELISA protocol has been modified by adding a solution of bovine serum albumin (0.5% in PBS) after the coating step.
UR - http://www.scopus.com/inward/record.url?scp=0027311619&partnerID=8YFLogxK
U2 - 10.1117/12.140237
DO - 10.1117/12.140237
M3 - Conference article
AN - SCOPUS:0027311619
SN - 0277-786X
VL - 1716
SP - 44
EP - 50
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
T2 - International Conference on Monitoring of Toxic Chemicals and Biomarkers 1992
Y2 - 15 June 1992 through 19 June 1992
ER -