TY - JOUR
T1 - Development of a stable isotope dilution assay for tenuazonic acid
AU - Asam, Stefan
AU - Liu, Yang
AU - Konitzer, Katharina
AU - Rychlik, Michael
PY - 2011/4/13
Y1 - 2011/4/13
N2 - A stable isotope dilution assay (SIDA) for the Alternaria mycotoxin tenuazonic acid was developed. Therefore, [13C6, 15N]-tenuazonic acid was synthesized from [13C 6,15N]-isoleucine by Dieckmann intramolecular cyclization after acetoacetylation with diketene. The synthesized [13C 6,15N]-tenuazonic acid was used as the internal standard for determination of tenuazonic acid in tomato products by liquid chromatography tandem mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine. Method validation revealed a limit of detection of 0.1 μg/kg and a limit of quantitation of 0.3 μg/kg. Recovery was close to 100% in the range of 3-300 μg/kg. Determination of tenuazonic acid in two samples of different tomato ketchups (naturally contaminated) was achieved with a coefficient of variation of 2.3% and 4.7%. Different tomato products (n = 16) were analyzed for their content of tenuazonic acid using the developed SIDA. Values were between 15 and 195 μg/kg (tomato ketchup, n = 9), 363 and 909 μg/kg (tomato paste, n = 2), and 8 and 247 μg/kg (pureed tomatoes and comparable products, n = 5).
AB - A stable isotope dilution assay (SIDA) for the Alternaria mycotoxin tenuazonic acid was developed. Therefore, [13C6, 15N]-tenuazonic acid was synthesized from [13C 6,15N]-isoleucine by Dieckmann intramolecular cyclization after acetoacetylation with diketene. The synthesized [13C 6,15N]-tenuazonic acid was used as the internal standard for determination of tenuazonic acid in tomato products by liquid chromatography tandem mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine. Method validation revealed a limit of detection of 0.1 μg/kg and a limit of quantitation of 0.3 μg/kg. Recovery was close to 100% in the range of 3-300 μg/kg. Determination of tenuazonic acid in two samples of different tomato ketchups (naturally contaminated) was achieved with a coefficient of variation of 2.3% and 4.7%. Different tomato products (n = 16) were analyzed for their content of tenuazonic acid using the developed SIDA. Values were between 15 and 195 μg/kg (tomato ketchup, n = 9), 363 and 909 μg/kg (tomato paste, n = 2), and 8 and 247 μg/kg (pureed tomatoes and comparable products, n = 5).
UR - http://www.scopus.com/inward/record.url?scp=79953903913&partnerID=8YFLogxK
U2 - 10.1021/jf104270e
DO - 10.1021/jf104270e
M3 - Article
C2 - 21370870
AN - SCOPUS:79953903913
SN - 0021-8561
VL - 59
SP - 2980
EP - 2987
JO - Journal of agricultural and food chemistry
JF - Journal of agricultural and food chemistry
IS - 7
ER -