Development of a highly sensitive enzyme-immunoassay for the determination of triazine herbicides

M. Winklmair, M. G. Weller, J. Mangler, B. Schlosshauer, R. Niessner

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59 Scopus citations


A monoclonal antibody (Mab) with extraordinary sensitivity and high class selectivity to triazine herbicides is described. With an enzyme-linked immunosorbent assay (ELISA) using Mab 4A54 IC50 values for terbuthylazine, atrazine, propazine and simazine below 0.1 μg/L (the EU maximum admissible concentration for individual pesticides) have been obtained. Detection limits of 0.004 μg/L for terbuthylazine, 0.006 μg/L for atrazine, 0.003 μg/L for propazine, 0.01 μg/L for simazine and 0.05 μg/L for deethylterbuthylazine could be achieved. Therefore, Mab 4A54 allows a sum screening of these five triazines in a relevant concentration range. To our knowledge, this is the most sensitive antibody to terbuthylazine at all and also the most sensitive Mab to all these four triazines. Another monoclonal antibody resulting from the same immunization, clone 4A118, exhibits best sensitivity for propazine (detection limit: 0.02 μg/L) at lower cross-reactivity to terbuthylazine and atrazine compared to clone 4A54. Affinity constants of both Mabs towards several triazines have been calculated. The application of both Mabs for the analysis of triazines in water samples of different origin has been tested and their resistance towards humic acid influence could be shown. A good correlation of the analysis of water samples with GC and ELISA was observed.

Original languageEnglish
Pages (from-to)614-622
Number of pages9
JournalFresenius' Journal of Analytical Chemistry
Issue number5
StatePublished - 1997


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