Development of a fluorescence polarization immunoassay for polycyclic aromatic hydrocarbons

Irina Yu Goryacheva, Sergei A. Eremin, Ekaterina A. Shutaleva, Miloslav Suchanek, Reinhard Niessner, Dietmar Knopp

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


A fluorescence polarization immunoassay for detection of polycyclic aromatic hydrocarbons (PAHs) was developed. Fluorescein-labeled tracers based on different PAHs were synthesized and studied in order to achieve a high sensitivity using both monoclonal and polyclonal antibodies. The application of different combinations of antibody tracer pairs allows to separately determine groups of small and large PAHs or to realize a class-specific assay. The limits of detection of PAHs were 0.9, 1.1, and 3.4 ng ml-1 for benzo[a]pyrene, naphthalene, and anthracene, respectively. The developed fluorescence polarization immunoassay could be used for rapid screening of natural water samples for contamination with PAHs.

Original languageEnglish
Pages (from-to)1445-1460
Number of pages16
JournalAnalytical Letters
Issue number7
StatePublished - Jan 2007


  • Fluorescence polarization immunoassay
  • PAHs
  • Screening


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