TY - JOUR
T1 - Development of a fluorescence polarization immunoassay for polycyclic aromatic hydrocarbons
AU - Goryacheva, Irina Yu
AU - Eremin, Sergei A.
AU - Shutaleva, Ekaterina A.
AU - Suchanek, Miloslav
AU - Niessner, Reinhard
AU - Knopp, Dietmar
N1 - Funding Information:
Institute of Hydrochemistry and Chemical Balneology, Technical University of Munich, München, Germany Abstract: A fluorescence polarization immunoassay for detection of polycyclic aromatic hydrocarbons (PAHs) was developed. Fluorescein-labeled tracers based on different PAHs were synthesized and studied in order to achieve a high sensitivity using both monoclonal and polyclonal antibodies. The application of different combinations of antibody tracer pairs allows to separately determine groups of small and large PAHs or to realize a class-specific assay. The limits of detection of PAHs were 0.9, 1.1, and 3.4 ng ml21 for benzo[a]pyrene, naphthalene, and anthracene, respect- Received 20 January 2007; accepted 27 February 2007 The financial supports were obtained from NATO grant “Development of express polarization fluoroimmunoassay of polycyclic aromatic hydrocarbons (PAHs) and its application for screening environmental samples” (EST.CLG.978618) and Grant from Programme EUREKA, OE77 COCANAL Address correspondence to Sergei A. Eremin, Department of Chemical Enzymology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia. E-mail: [email protected]
PY - 2007/1
Y1 - 2007/1
N2 - A fluorescence polarization immunoassay for detection of polycyclic aromatic hydrocarbons (PAHs) was developed. Fluorescein-labeled tracers based on different PAHs were synthesized and studied in order to achieve a high sensitivity using both monoclonal and polyclonal antibodies. The application of different combinations of antibody tracer pairs allows to separately determine groups of small and large PAHs or to realize a class-specific assay. The limits of detection of PAHs were 0.9, 1.1, and 3.4 ng ml-1 for benzo[a]pyrene, naphthalene, and anthracene, respectively. The developed fluorescence polarization immunoassay could be used for rapid screening of natural water samples for contamination with PAHs.
AB - A fluorescence polarization immunoassay for detection of polycyclic aromatic hydrocarbons (PAHs) was developed. Fluorescein-labeled tracers based on different PAHs were synthesized and studied in order to achieve a high sensitivity using both monoclonal and polyclonal antibodies. The application of different combinations of antibody tracer pairs allows to separately determine groups of small and large PAHs or to realize a class-specific assay. The limits of detection of PAHs were 0.9, 1.1, and 3.4 ng ml-1 for benzo[a]pyrene, naphthalene, and anthracene, respectively. The developed fluorescence polarization immunoassay could be used for rapid screening of natural water samples for contamination with PAHs.
KW - Fluorescence polarization immunoassay
KW - PAHs
KW - Screening
UR - http://www.scopus.com/inward/record.url?scp=34249880461&partnerID=8YFLogxK
U2 - 10.1080/00032710701297034
DO - 10.1080/00032710701297034
M3 - Article
AN - SCOPUS:34249880461
SN - 0003-2719
VL - 40
SP - 1445
EP - 1460
JO - Analytical Letters
JF - Analytical Letters
IS - 7
ER -