Development of a fast and sensitive UPLC-MS/MS method for quantitation of dilignols in aged garlic extract

Toshiaki Matsutomo, Timo D. Stark, Thomas Hofmann

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A rapid, sensitive, and specific liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantitation of the dilignols (–)-(2R,3S)-dihydrodehydrodiconiferyl alcohol (1), (+)-(2S,3R)-dehydrodiconiferyl alcohol (2), erythro-guaiacylglycerol-β-O-4′-coniferyl ether (3), and threo-guaiacylglycerol-β-O-4′-coniferyl ether (4) which have been previously identified as key antioxidants in aged garlic extract. After optimization of the MS conditions, the dilignols were analyzed by means of the multiple reaction monitoring, and their quantitation was validated. The calibration curves showed good linearities (R2 > 0.999), and the LOQ were 0.1–5.9 ng/mL.

Original languageEnglish
Pages (from-to)849-854
Number of pages6
JournalEuropean Food Research and Technology
Volume242
Issue number6
DOIs
StatePublished - 1 Jun 2016

Keywords

  • Aged garlic extract
  • Dilignol
  • UPLC-MS/MS

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