@article{c5c01333b7714dc5b048c9b3bb99175e,
title = "Development of a capillary isoelectric focusing immunoassay to measure DJ-1 isoforms in biological samples",
abstract = "We report on the development of a novel assay protocol for the separation and detection of charge isoforms of DJ-1 in biological samples by automated capillary isoelectric focusing followed by immunological detection. DJ-1 (PARK7) is considered as a biomarker candidate for Parkinson's disease and may potentially support the differentiation of clinical subtypes of the disease. The new method allows for separation and subsequent relative quantitative comparison of different isoforms of DJ-1 in biological samples. The assay was successfully applied to the analysis of DJ-1 isoform patterns in brains from mice subjected to normal or high-fat diet and revealed statistically significant group differences. Furthermore, in a pooled and concentrated sample of human cerebrospinal fluid that was depleted of albumin and immunoglobulin G, four different charge variants of DJ-1 could be detected. Taken together, the capillary isoelectric focusing immunoassay for DJ-1 represents a promising tool that may ultimately serve in clinical biomarker studies.",
keywords = "Biomarker, CSF, DJ-1, Immunoassay, Isoelectric focusing, PARK7",
author = "{Besong Agbo}, D. and H. Klafki and G. Poschmann and K. Seyfarth and J. Genius and C. Jan{\ss}en and K. St{\"u}hler and W. Wurst and Meyer, {H. E.} and M. Klingenspor and J. Wiltfang",
note = "Funding Information: This work was supported by the FP7 EU grant NADINE (grant agreement 246513), by the German Federal Ministry for Education and Research (BioPharma–Neuroallianz grant 161A120B/031A120B), and by the grant PURE (Protein Research Unit Ruhr within Europe) from the state government of North Rhine–Westphalia and is a part of the BIOMARKAPD project in the JPND program. K.S. was funded by the Federal Ministry of Education and Research (grant NGFN-PLUS, PHF-01GS08195-3b), M.K. was funded by the Federal Ministry of Education and Research (NGFNplus 01GS0822), and G.P. was funded by the Research Commission of the Medical Faculty of the Heinrich Heine University D{\"u}sseldorf. This work was also supported by the German Ministry of Education and Research within the DIGTOP project (grant 01GS0858 to W.W.) and by the NGFNPlus Program Parkinson (grant 01GS08174 to W.W.). ",
year = "2013",
doi = "10.1016/j.ab.2013.09.013",
language = "English",
volume = "443",
pages = "197--204",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",
}