Development of a Bioprocess for the Production of Cyclic Lipopeptides Pseudofactins With Efficient Purification From Collected Foam

Piotr Biniarz, Marius Henkel, Rudolf Hausmann, Marcin Łukaszewicz

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Microbial surfactants (biosurfactants) have gained interest as promising substitutes of synthetic surface-active compounds. However, their production and purification are still challenging, with significant room for efficiency and costs optimization. In this work, we introduce a method for the enhanced production and purification of cyclic lipopeptides pseudofactins (PFs) from Pseudomonas fluorescens BD5 cultures. The method is directly applicable in a technical scale with the possibility of further upscaling. Comparing to the original protocol for production of PFs (cultures in mineral salt medium in shaken flasks followed by solvent-solvent extraction of PFs), our process offers not only ∼24-fold increased productivity, but also easier and more efficient purification. The new process combines high yield of PFs (∼7.2 grams of PFs per 30 L of working volume), with recovery levels of 80–90% and purity of raw PFs up to 60–70%. These were achieved with an innovative, single-step thermal co-precipitation and extraction of PFs directly from collected foam, as a large amount of PF-enriched foam was produced during the bioprocess. Besides we present a protocol for the selective production of PF structural analogs and their separation with high-performance liquid chromatography. Our approach can be potentially utilized in the efficient production and purification of other lipopeptides of Pseudomonas and Bacillus origin.

Original languageEnglish
Article number565619
JournalFrontiers in Bioengineering and Biotechnology
Volume8
DOIs
StatePublished - 23 Nov 2020
Externally publishedYes

Keywords

  • Pseudomonas fluorescens
  • bioreactor
  • biosurfactant production
  • cyclic lipopeptides (CLPs)
  • foam fractionation
  • lipopeptide production
  • lipopeptide purification

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