TY - JOUR
T1 - Development and application of a method for the analysis of N-acylhomoserine lactones by solid-phase extraction and ultra high pressure liquid chromatography
AU - Li, Xiaojing
AU - Fekete, Agnes
AU - Englmann, Matthias
AU - Götz, Christine
AU - Rothballer, Michael
AU - Frommberger, Moritz
AU - Buddrus, Katharina
AU - Fekete, Jenoe
AU - Cai, Chunping
AU - Schröder, Peter
AU - Hartmann, Anton
AU - Chen, Guonan
AU - Schmitt-Kopplin, Philippe
N1 - Funding Information:
All work was done at the GSF National Research Center for Environment and Health with financial support of Chinese Scholarship Council (CSC) of X.L. as guest scientist. Special thanks Brigitte Look and Heidi Neumeir for their precise technical assistance as well as for their always encouraging words.
PY - 2006/11/17
Y1 - 2006/11/17
N2 - A robust method based on solid-phase extraction (SPE) followed by ultra high pressure liquid chromatography (with trade name of Ultra Performance Liquid Chromatography: UPLC; Waters, Milford, MA, USA) is proposed for the determination of five derivatives of N-acylhomoserine lactones (AHLs) that play a biological role as signal molecules of several gram-negative bacteria. Different commercial SPE cartridges were tested for sample extraction, clean-up and preconcentration. Since the sample matrix was a complex growth media, careful optimization of the SPE with respect to washing procedure, elution solvent and sample solvent was necessary. No sample loss was observed when up to 100 mL spiked full media was added onto the cartridge. Applying UPLC for the determination of AHLs, the performance characteristics of the method showed good separation efficiency and high speed. In order to demonstrate the applicability of the method, supernatants with the known AHL producer Burkholderia cepacia LA3 grown in different media were investigated. Additionally, the method was successfully used for the degradation/uptake study of AHLs from a liquid matrix in which barley was grown under controlled condition.
AB - A robust method based on solid-phase extraction (SPE) followed by ultra high pressure liquid chromatography (with trade name of Ultra Performance Liquid Chromatography: UPLC; Waters, Milford, MA, USA) is proposed for the determination of five derivatives of N-acylhomoserine lactones (AHLs) that play a biological role as signal molecules of several gram-negative bacteria. Different commercial SPE cartridges were tested for sample extraction, clean-up and preconcentration. Since the sample matrix was a complex growth media, careful optimization of the SPE with respect to washing procedure, elution solvent and sample solvent was necessary. No sample loss was observed when up to 100 mL spiked full media was added onto the cartridge. Applying UPLC for the determination of AHLs, the performance characteristics of the method showed good separation efficiency and high speed. In order to demonstrate the applicability of the method, supernatants with the known AHL producer Burkholderia cepacia LA3 grown in different media were investigated. Additionally, the method was successfully used for the degradation/uptake study of AHLs from a liquid matrix in which barley was grown under controlled condition.
KW - Method development
KW - N-Acylhomoserine lactones
KW - Quorum sensing
KW - Solid-phase extraction
KW - UPLC
UR - http://www.scopus.com/inward/record.url?scp=33750319330&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2006.09.047
DO - 10.1016/j.chroma.2006.09.047
M3 - Article
C2 - 17049538
AN - SCOPUS:33750319330
SN - 0021-9673
VL - 1134
SP - 186
EP - 193
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -