Abstract
Sensitive enzyme immunoassay for the determination of total leukocyte neutral proteinase inhibitor and polymorphonuclear elastase-leukocyte neutral proteinase inhibitor complexes are described. The usable ranges of the standard curves were from 80 ng/l to 10 μg/l. The relative intra-assay coefficients of variation of the tests were between 2 and 4%, and the inter-assay coefficients of variation between 4 and 10%. In vitro and in vivo studies were performed with septic pigs and isolated leukocytes. The results show that leukocyte neutral proteinase inhibitor can be used in pigs as a parameter for the development of a septicaemia.
| Original language | English |
|---|---|
| Pages (from-to) | 605-609 |
| Number of pages | 5 |
| Journal | Journal of Clinical Chemistry and Clinical Biochemistry |
| Volume | 26 |
| Issue number | 10 |
| State | Published - 1988 |
| Externally published | Yes |