Abstract
A microquantitative procedure is described to determine bile acids in 5-15 mg liver tissue, which can be obtained from the specimen of needle biopsy performed for histological examination. The tissue cylinder is solubilized in KOH and the bile acids are deconjugated by cholylglycine hydrolase, purified by thin-layer chromatography, methylated, and trimethylsililated for gas chromatographic separation and quantitation. Recovery of taurine- and glycine-conjugated bile acid standards is 73-86%; the experimental error of the procedure is less than 15%. The technique permits investigation of hepatic bile acids in liver diseases without requiring surgical exploration.
Original language | English |
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Pages (from-to) | 280-286 |
Number of pages | 7 |
Journal | Biochemical medicine |
Volume | 8 |
Issue number | 2 |
DOIs | |
State | Published - Oct 1973 |
Externally published | Yes |