Determination of Atrazine Residues in Electro‐Ultrafiltration Soil Extracts by a Sensitive Enzyme Immunoassay

Norbert Staimer; Klaus Bürcky; Georg Steinle; Bertold Hock

doi:10.1002/jpln.19941570108

Determination of Atrazine Residues in Electro‐Ultrafiltration Soil Extracts by a Sensitive Enzyme Immunoassay

Norbert Staimer
, Klaus Bürcky
, Georg Steinle
, Bertold Hock

Life Sciences

Technical University of Munich
BGD - Bodengesundheitsdienst GmbH
Südzucker Ag
Entwicklung Und Services

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A sensitive enzyme immunoassay (EIA) based on polyclonal antibodies from sheep was used to screen for atrazine in electro‐ultrafiltration (EUF) soil extracts without clean‐up. Matrix effects were circumvented by diluting the aqueous EUF extracts. The EUF proved to be a convenient method for the extraction of atrazine residues in soil. The efficiency of EUF appeared to be equivalent to that of organic extraction methods except on weathered residues, which generally resulted in lower yields. Both the combined gas chromatography/automated Soxhlet (GC/Soxtec) and the immunochemical technique EIA/EUF yielded similar data for the 26 soil samples identified as positive (> 0.02 mg/kg) during the first screening of 479 EUF extracts by the EIA.

Original language	English
Pages (from-to)	41-46
Number of pages	6
Journal	Zeitschrift für Pflanzenernährung und Bodenkunde
Volume	157
Issue number	1
DOIs	https://doi.org/10.1002/jpln.19941570108
State	Published - 1994

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abstract = "A sensitive enzyme immunoassay (EIA) based on polyclonal antibodies from sheep was used to screen for atrazine in electro‐ultrafiltration (EUF) soil extracts without clean‐up. Matrix effects were circumvented by diluting the aqueous EUF extracts. The EUF proved to be a convenient method for the extraction of atrazine residues in soil. The efficiency of EUF appeared to be equivalent to that of organic extraction methods except on weathered residues, which generally resulted in lower yields. Both the combined gas chromatography/automated Soxhlet (GC/Soxtec) and the immunochemical technique EIA/EUF yielded similar data for the 26 soil samples identified as positive (> 0.02 mg/kg) during the first screening of 479 EUF extracts by the EIA.",

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AU - Hock, Bertold

PY - 1994

Y1 - 1994

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Determination of Atrazine Residues in Electro‐Ultrafiltration Soil Extracts by a Sensitive Enzyme Immunoassay

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