Abstract
An enzyme-linked immunosorbent assay (ELISA) on microplates and a HPLC coupled-column switching method were compared for the determination of the nitroarene compound I-nitropyrene in airborne particulate organic matter collected at a busy intersection over a period of 2 months. After purification of the sample extract with silica, in the multidimensional chromatographic method nitroarenes were separated on a RP18 precolumn from matrix constituents followed by on-line reduction to corresponding aminoarenes with a Pt catalyst on alumina and a further separation of 1- aminopyrene on a second RP18 column. Methanol-water (70:30, v/v) was the mobile phase used. With ELISA, a six-fold overestimation was obtained for untreated samples. After clean-up it was lowered to ~1.6-fold overestimation which was mainly caused by cross-reaction of 2-nitropyrene and 2- nitrofluoranthene.
Original language | English |
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Pages (from-to) | 209-217 |
Number of pages | 9 |
Journal | Journal of Chromatography A |
Volume | 807 |
Issue number | 2 |
DOIs | |
State | Published - 22 May 1998 |
Keywords
- Diesel soot
- I-Nitropyrene