Detection of tick-borne encephalitis virus RNA in ticks (Ixodes ricinus) by the polymerase chain reaction

Christiane Ramelow, Jochen Süss, Doris Berndt, Michael Roggendorf, Eckart Schreier

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

A polymerase chain reaction for the detection of tick-borne encephalitis virus (TBEV) RNA in ticks was developed. Two pairs of primers for nested PCR were selected from the 5'-NCR and the 5'-terminus of the C protein coding region, which are highly conserved among the TBEV isolates sequenced so far. The sensitivity of the nested PCR was tested by dilution experiments of a TBEV positive brain suspension. The specificity of the PCR products was confirmed by Southern blotting. In a pilot study, 60 homogenates of 7200 ticks (I. ricinus) were examined by PCR. Two homogenates were found positive. The PCR for TBEV RNA appears to be a valuable method to define endemic areas of TBE.

Original languageEnglish
Pages (from-to)115-119
Number of pages5
JournalJournal of Virological Methods
Volume45
Issue number1
DOIs
StatePublished - Nov 1993
Externally publishedYes

Keywords

  • Flaviviridae
  • PCR
  • Southern blotting
  • Tick-borne encephalitis virus

Fingerprint

Dive into the research topics of 'Detection of tick-borne encephalitis virus RNA in ticks (Ixodes ricinus) by the polymerase chain reaction'. Together they form a unique fingerprint.

Cite this