Abstract
A method for the detection of the genetic modification in thermally treated products from insect-resistant maize expressing a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis is described. The probability of detection of the transgene in thermally treated products was increased by choosing a short (211 bp) polymerase chain reaction (PCR) amplicon; the specificity was ensured by including a promoter region adjacent to the cryIA(b) gene in the target sequence. The influence of pH during thermal treatment of maize on the detectability of the transgene is demonstrated using polenta as a model for products made from this cereal.
| Original language | English |
|---|---|
| Pages (from-to) | 203-207 |
| Number of pages | 5 |
| Journal | Zeitschrift fur Lebensmittel -Untersuchung und -Forschung |
| Volume | 206 |
| Issue number | 3 |
| State | Published - 1998 |
Keywords
- Bacillus thuringiensis
- Genetically modified maize
- Polymerase chain reaction
- Thermal treatment