Detection of lipid bilayer and peptide pore formation at gigahertz frequencies

Michael Olapinski; Stephan Manus; Michael George; Andrea Brüggemann; Niels Fertig; Friedrich C. Simmel

doi:10.1063/1.2159571

Detection of lipid bilayer and peptide pore formation at gigahertz frequencies

Michael Olapinski, Stephan Manus, Michael George, Andrea Brüggemann, Niels Fertig, Friedrich C. Simmel

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A coplanar waveguide defined on a borosilicate glass chip is integrated with a microfluidic cartridge to allow for characterization of lipid bilayer membranes at gigahertz frequencies. The membranes are deposited on the waveguide using a vesicle fusion method. The deposition of the bilayers results in a significant change in the high-frequency transmission properties of the waveguide. We also embed alamethicin, an ion channel forming peptide, in the bilayers. The influence of the pore forming peptides can be clearly detected in the transmission signal.

Original language	English
Article number	013902
Journal	Applied Physics Letters
Volume	88
Issue number	1
DOIs	https://doi.org/10.1063/1.2159571
State	Published - 2006
Externally published	Yes

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title = "Detection of lipid bilayer and peptide pore formation at gigahertz frequencies",

abstract = "A coplanar waveguide defined on a borosilicate glass chip is integrated with a microfluidic cartridge to allow for characterization of lipid bilayer membranes at gigahertz frequencies. The membranes are deposited on the waveguide using a vesicle fusion method. The deposition of the bilayers results in a significant change in the high-frequency transmission properties of the waveguide. We also embed alamethicin, an ion channel forming peptide, in the bilayers. The influence of the pore forming peptides can be clearly detected in the transmission signal.",

author = "Michael Olapinski and Stephan Manus and Michael George and Andrea Br{\"u}ggemann and Niels Fertig and Simmel, {Friedrich C.}",

note = "Funding Information: The authors thank J. P. Kotthaus and R. H. Blick for initial input and many useful discussions and J. O. R{\"a}dler and his lab for help in the preparation of lipid bilayers. This work was supported by the Bundesministerium f{\"u}r Bildung und Forschung (BMBF Nos. 13N8363 and 13N8364) and the Deutsche Forschungsgemeinschaft (DFG SI 761/2-2).",

year = "2006",

doi = "10.1063/1.2159571",

language = "English",

volume = "88",

journal = "Applied Physics Letters",

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T1 - Detection of lipid bilayer and peptide pore formation at gigahertz frequencies

AU - Olapinski, Michael

AU - Manus, Stephan

AU - George, Michael

AU - Brüggemann, Andrea

AU - Fertig, Niels

AU - Simmel, Friedrich C.

N1 - Funding Information: The authors thank J. P. Kotthaus and R. H. Blick for initial input and many useful discussions and J. O. Rädler and his lab for help in the preparation of lipid bilayers. This work was supported by the Bundesministerium für Bildung und Forschung (BMBF Nos. 13N8363 and 13N8364) and the Deutsche Forschungsgemeinschaft (DFG SI 761/2-2).

PY - 2006

Y1 - 2006

N2 - A coplanar waveguide defined on a borosilicate glass chip is integrated with a microfluidic cartridge to allow for characterization of lipid bilayer membranes at gigahertz frequencies. The membranes are deposited on the waveguide using a vesicle fusion method. The deposition of the bilayers results in a significant change in the high-frequency transmission properties of the waveguide. We also embed alamethicin, an ion channel forming peptide, in the bilayers. The influence of the pore forming peptides can be clearly detected in the transmission signal.

AB - A coplanar waveguide defined on a borosilicate glass chip is integrated with a microfluidic cartridge to allow for characterization of lipid bilayer membranes at gigahertz frequencies. The membranes are deposited on the waveguide using a vesicle fusion method. The deposition of the bilayers results in a significant change in the high-frequency transmission properties of the waveguide. We also embed alamethicin, an ion channel forming peptide, in the bilayers. The influence of the pore forming peptides can be clearly detected in the transmission signal.

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DO - 10.1063/1.2159571

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JO - Applied Physics Letters

JF - Applied Physics Letters

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Detection of lipid bilayer and peptide pore formation at gigahertz frequencies

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