Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

Markus T. Berninger; Pouyan Mohajerani; Moritz Wildgruber; Nicolas Beziere; Melanie A. Kimm; Xiaopeng Ma; Bernhard Haller; Megan J. Fleming; Stephan Vogt; Martina Anton; Andreas B. Imhoff; Vasilis Ntziachristos; Reinhard Meier; Tobias D. Henning

doi:10.1016/j.pacs.2017.04.002

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

Markus T. Berninger
, Pouyan Mohajerani
, Moritz Wildgruber
, Nicolas Beziere
, Melanie A. Kimm
, Xiaopeng Ma
, Bernhard Haller
, Megan J. Fleming
, Stephan Vogt
, Martina Anton
, Andreas B. Imhoff
, Vasilis Ntziachristos
, Reinhard Meier
, Tobias D. Henning

Associate Professorship of Sports Orthopaedy

Technical University of Munich
Berufsgenossenschaftliche Unfallklinik Murnau
Helmholtz Zentrum München German Research Center for Environmental Health
University Hospital of Cologne

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 10⁶, 2 × 10⁵ and 2 × 10⁴ MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.

Original language	English
Pages (from-to)	37-47
Number of pages	11
Journal	Photoacoustics
Volume	6
DOIs	https://doi.org/10.1016/j.pacs.2017.04.002
State	Published - 1 Jun 2017

Keywords

Cell labeling
Fluorescence molecular imaging
Intramyocardial injection
Mesenchymal stem cells
Multispectral optoacoustic tomography
Rabbit heart

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10.1016/j.pacs.2017.04.002

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Berninger, M. T., Mohajerani, P., Wildgruber, M., Beziere, N., Kimm, M. A., Ma, X., Haller, B., Fleming, M. J., Vogt, S., Anton, M., Imhoff, A. B., Ntziachristos, V., Meier, R., & Henning, T. D. (2017). Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography. Photoacoustics, 6, 37-47. https://doi.org/10.1016/j.pacs.2017.04.002

@article{5514d0def0174fb0b1030dc129711f96,

title = "Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography",

abstract = "The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.",

keywords = "Cell labeling, Fluorescence molecular imaging, Intramyocardial injection, Mesenchymal stem cells, Multispectral optoacoustic tomography, Rabbit heart",

author = "Berninger, \{Markus T.\} and Pouyan Mohajerani and Moritz Wildgruber and Nicolas Beziere and Kimm, \{Melanie A.\} and Xiaopeng Ma and Bernhard Haller and Fleming, \{Megan J.\} and Stephan Vogt and Martina Anton and Imhoff, \{Andreas B.\} and Vasilis Ntziachristos and Reinhard Meier and Henning, \{Tobias D.\}",

note = "Publisher Copyright: {\textcopyright} 2017",

year = "2017",

month = jun,

day = "1",

doi = "10.1016/j.pacs.2017.04.002",

language = "English",

volume = "6",

pages = "37--47",

journal = "Photoacoustics",

issn = "2213-5979",

publisher = "Elsevier GmbH",

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Berninger, MT, Mohajerani, P, Wildgruber, M, Beziere, N, Kimm, MA, Ma, X, Haller, B, Fleming, MJ, Vogt, S, Anton, M, Imhoff, AB , Ntziachristos, V, Meier, R & Henning, TD 2017, 'Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography', Photoacoustics, vol. 6, pp. 37-47. https://doi.org/10.1016/j.pacs.2017.04.002

TY - JOUR

T1 - Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

AU - Berninger, Markus T.

AU - Mohajerani, Pouyan

AU - Wildgruber, Moritz

AU - Beziere, Nicolas

AU - Kimm, Melanie A.

AU - Ma, Xiaopeng

AU - Haller, Bernhard

AU - Fleming, Megan J.

AU - Vogt, Stephan

AU - Anton, Martina

AU - Imhoff, Andreas B.

AU - Ntziachristos, Vasilis

AU - Meier, Reinhard

AU - Henning, Tobias D.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.

AB - The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.

KW - Cell labeling

KW - Fluorescence molecular imaging

KW - Intramyocardial injection

KW - Mesenchymal stem cells

KW - Multispectral optoacoustic tomography

KW - Rabbit heart

UR - https://www.scopus.com/pages/publications/85019168660

U2 - 10.1016/j.pacs.2017.04.002

DO - 10.1016/j.pacs.2017.04.002

M3 - Article

AN - SCOPUS:85019168660

SN - 2213-5979

VL - 6

SP - 37

EP - 47

JO - Photoacoustics

JF - Photoacoustics

ER -

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

Abstract

Keywords

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