Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

Markus T. Berninger, Pouyan Mohajerani, Moritz Wildgruber, Nicolas Beziere, Melanie A. Kimm, Xiaopeng Ma, Bernhard Haller, Megan J. Fleming, Stephan Vogt, Martina Anton, Andreas B. Imhoff, Vasilis Ntziachristos, Reinhard Meier, Tobias D. Henning

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.

Original languageEnglish
Pages (from-to)37-47
Number of pages11
JournalPhotoacoustics
Volume6
DOIs
StatePublished - 1 Jun 2017

Keywords

  • Cell labeling
  • Fluorescence molecular imaging
  • Intramyocardial injection
  • Mesenchymal stem cells
  • Multispectral optoacoustic tomography
  • Rabbit heart

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