TY - JOUR
T1 - Detection of genetically modified insect-resistant Bt maize by means of polymerase chain reaction
AU - Hupfer, Christine
AU - Hotzel, Helmut
AU - Sachse, Konrad
AU - Engel, Karl Heinz
PY - 1997
Y1 - 1997
N2 - A method for the detection of genetically modified insect-resistant maize, expressing a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis is described. The procedure includes: (1) extraction of genomic DNA, (2) amplification of the inserted synthetic gene by means of the polymerase chain reaction (PCR) method, and (3) characterization and confirmation of the PCR product by Southern hybridization with a digoxigenin-labelled oligonucleotide probe and DNA sequencing.
AB - A method for the detection of genetically modified insect-resistant maize, expressing a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis is described. The procedure includes: (1) extraction of genomic DNA, (2) amplification of the inserted synthetic gene by means of the polymerase chain reaction (PCR) method, and (3) characterization and confirmation of the PCR product by Southern hybridization with a digoxigenin-labelled oligonucleotide probe and DNA sequencing.
KW - Bacillus thuringiensis
KW - Genetically modified maize
KW - Insect-resistant maize
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=0001101861&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0001101861
SN - 0044-3026
VL - 205
SP - 442
EP - 445
JO - Zeitschrift fur Lebensmittel -Untersuchung und -Forschung
JF - Zeitschrift fur Lebensmittel -Untersuchung und -Forschung
IS - 6
ER -