TY - JOUR
T1 - Detection of experimental myocardium infarction in rats by MRI using heat shock protein 70 conjugated superparamagnetic iron oxide nanoparticle
AU - Shevtsov, Maxim A.
AU - Nikolaev, Boris P.
AU - Ryzhov, Vyacheslav A.
AU - Yakovleva, Ludmila Y.
AU - Dobrodumov, Anatolii V.
AU - Marchenko, Yaroslav Y.
AU - Margulis, Boris A.
AU - Pitkin, Emil
AU - Mikhrina, Anastasiy L.
AU - Guzhova, Irina V.
AU - Multhoff, Gabriele
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Superparamagnetic iron-oxide based contrast agents can provide important diagnostic information regarding the assessment of cardiac inflammatory diseases. The aim of the study was to analyze whether nanoparticles conjugated to recombinant 70-kDa heat shock protein (Hsp70-SPION) can be applied for the detection of acute myocardium infarct by MRI. Cellular experiments demonstrated increased CD40-mediated uptake of Hsp70-SPIONs in comparison to non-conjugated SPIONs. Following induction of an acute infarct in rats by ligation of the left anterior descending artery SPIONs and Hsp70-SPION conjugates were injected intravenously on day 4. The animals underwent sequential MRI that showed the presence of the particles in the infarcted zone. Subsequent biodistribution analyses with the help of method on non-linear magnetic response indicated the preferential accumulation of the Hsp70-SPIONs in the heart tissue that was further confirmed with histological analyses. The study demonstrated that an acute infarct can be visualized by MRI using Hsp70-functionalized SPION conjugates.
AB - Superparamagnetic iron-oxide based contrast agents can provide important diagnostic information regarding the assessment of cardiac inflammatory diseases. The aim of the study was to analyze whether nanoparticles conjugated to recombinant 70-kDa heat shock protein (Hsp70-SPION) can be applied for the detection of acute myocardium infarct by MRI. Cellular experiments demonstrated increased CD40-mediated uptake of Hsp70-SPIONs in comparison to non-conjugated SPIONs. Following induction of an acute infarct in rats by ligation of the left anterior descending artery SPIONs and Hsp70-SPION conjugates were injected intravenously on day 4. The animals underwent sequential MRI that showed the presence of the particles in the infarcted zone. Subsequent biodistribution analyses with the help of method on non-linear magnetic response indicated the preferential accumulation of the Hsp70-SPIONs in the heart tissue that was further confirmed with histological analyses. The study demonstrated that an acute infarct can be visualized by MRI using Hsp70-functionalized SPION conjugates.
KW - CD40
KW - Hsp70
KW - Magnetic resonance imaging
KW - Myocardial infarction
KW - Superparamagnetic nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84959419944&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2015.10.017
DO - 10.1016/j.nano.2015.10.017
M3 - Article
C2 - 26656626
AN - SCOPUS:84959419944
SN - 1549-9634
VL - 12
SP - 611
EP - 621
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
IS - 3
ER -