Detection of erwinia amylovora cells in apple shoots

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

For epidemiology and disease control it is important to know the dissemination of the pathogens in the plants. Propagation material for grafting could not be tested for latent infections by the fire blight pathogen till now. For proving the presence of Erwinia amylovora it is necessary to get and to test the sap of the suspected shoots. A simple new technique for extracting plant sap from woody shoots is described. For this purpose, shoots must be cutted in 10 to 30 cm long segments. Then one end of every segment is pulled over by a plastic tubing and airtightly connected with a vacuum pump. The other end of the segment must be submerged into a vessel filled with sterile water. In this way it was possible to get plant sap, more and faster from the basis and the middle of the shoots, than from their upper parts and less woody tops. In the sap of shoots of high susceptible varieties, inoculated in autumn 1997 and developed only short tip necroses, we could detect E. amylovora cells in spring 1998. In the sap of shoots of the resistant variety 'Retina' we couldn't find cells of that pathogen.

Original languageEnglish
Title of host publicationVIII International Workshop on Fire Blight
PublisherInternational Society for Horticultural Science
Pages511-514
Number of pages4
ISBN (Print)9789066059214
DOIs
StatePublished - 1999
Externally publishedYes

Publication series

NameActa Horticulturae
Volume489
ISSN (Print)0567-7572

Keywords

  • Erwinia amylovora
  • Extraction
  • Plant sap
  • Woody plant parts

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