Detection of chlamydia trachomatis by isolation in cell culture and enzyme amplified immunoassay

T. F. Schwarz, G. Zoulek, M. Roggendorf

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Abstract

188 (139 ♀ ; 49 ♂) genital swabs from patients with urogenital complaints (> 6 weeks), 69 (33 ♀ ; 36 ♂) conjunctival swabs from patients with chronic conjunctivitis and 14 swabs from newborns with acute conjunctivitis were tested for the presence of Chlamydia trachomatis (Ct) by inoculation in cell culture and visualisation by indirect immunfluorescence (IFT) with a monoclonal antibody and enzyme immunoassay (ELISA). Of the 271 specimens 20 (7.4%) were Ct positive by cell culture (IFT) and 18 (6.6%) were positive by ELISA. In 184 (97.9%) urogenital specimens results between cell culture (IFT) and ELISA agreed. With ELISA 4 further genital specimens were found to be Ct positive. In 59 (85.5%) conjunctival swabs of patients with chronic conjunctivitis results between cell culture (IFT) and ELISA agreed. By inoculation of cell culture (IFT) 6 (8.7%) more Ct positive specimens could be identified which were negative in ELISA. There was complete agreement between positive and negative Ct detection with cell culture (IFT) and ELISA in the cases of acute conjunctivitis.

Original languageEnglish
Pages (from-to)341-345
Number of pages5
JournalZentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A
Volume269
Issue number3
DOIs
StatePublished - 1988
Externally publishedYes

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