@article{d2d85b25cc5e4c029e4341ed250a81aa,
title = "Detection and delineation of oral cancer with a PARP1 targeted optical imaging agent",
abstract = "Earlier and more accurate detection of oral squamous cell carcinoma (OSCC) is essential to improve the prognosis of patients and to reduce the morbidity of surgical therapy. Here, we demonstrate that the nuclear enzyme Poly(ADP-ribose)Polymerase 1 (PARP1) is a promising target for optical imaging of OSCC with the fluorescent dye PARPi-FL. In patient-derived OSCC specimens, PARP1 expression was increased 7.8 ± 2.6-fold when compared to normal tissue. Intravenous injection of PARPi-FL allowed for high contrast in vivo imaging of human OSCC models in mice with a surgical fluorescence stereoscope and high-resolution imaging systems. The emitted signal was specific for PARP1 expression and, most importantly, PARPi-FL can be used as a topical imaging agent, spatially resolving the orthotopic tongue tumors in vivo. Collectively, our results suggest that PARP1 imaging with PARPi-FL can enhance the detection of oral cancer, serve as a screening tool and help to guide surgical resections.",
author = "Susanne Kossatz and Christian Brand and Stanley Gutiontov and Liu, {Jonathan T.C.} and Lee, {Nancy Y.} and Mithat Gonen and Weber, {Wolfgang A.} and Thomas Reiner",
note = "Funding Information: The authors thank Dr. Jason S. Lewis for helpful discussions. The authors further thank the Small-Animal Imaging Core Facility and the Molecular Cytology Core at Memorial Sloan Kettering Cancer Center (P30 CA008748). This work was funded in part by K25 EB016673 (T.R.) and R01 DE023497 (J.T.C.L.), the Brain Tumor Center of Memorial Sloan Kettering Cancer Center, and the Imaging and Radiation Sciences Program. Susanne Kossatz was funded by a scholarship from the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG, reference number KO 4966/1-1). The authors also wish to thank Dr. Nadeem Riaz and Brett Yarusi for help with human biospecimens, and Nora Katabi for help with pathology. We thank Steven Y. Leigh and Danni Wang for help with acquiring dual-axis microscope imagery.",
year = "2016",
month = feb,
day = "22",
doi = "10.1038/srep21371",
language = "English",
volume = "6",
journal = "Scientific Reports",
publisher = "Nature Publishing Group",
}