Detection and characterization of hepatitis C virus sequence in the serum of a patient with chronic HCV infection.

E. Schreier, K. Fuchs, M. Höhne, M. Motz, R. Zachoval, J. Esteban, S. Dittmann, F. Deinhardt, M. Roggendorf

Research output: Contribution to journalArticlepeer-review

Abstract

A cDNA fragment corresponding to the nonstructural gene region of Hepatitis C virus was cloned and sequenced. cDNA was obtained by reverse transcription of viral RNA extracted from serum of a German patient with chronic post transfusion hepatitis. "Nested" PCR resulted in a cDNA fragment of 345 nt. The sequence showed a homology of 96% to the American prototype HCV.

Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalArchives of Virology, Supplement
Volume4
DOIs
StatePublished - 1992
Externally publishedYes

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