Abstract
Nanotechnology and synthetic biology are rapidly converging, with DNA origami being one of the leading bridging technologies. DNA origami was shown to work well in a wide array of biotic environments. However, the large majority of extant DNA origami scaffolds utilize bacteriophages or plasmid sequences thus severely limiting its future applicability as a bio-orthogonal nanotechnology platform. In this paper we present the design of biologically inert (i.e., "bio-orthogonal") origami scaffolds. The synthetic scaffolds have the additional advantage of being uniquely addressable (unlike biologically derived ones) and hence are better optimized for high-yield folding. We demonstrate our fully synthetic scaffold design with both DNA and RNA origamis and describe a protocol to produce these bio-orthogonal and uniquely addressable origami scaffolds.
Original language | English |
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Pages (from-to) | 1140-1149 |
Number of pages | 10 |
Journal | ACS Synthetic Biology |
Volume | 6 |
Issue number | 7 |
DOIs | |
State | Published - 21 Jul 2017 |
Externally published | Yes |
Keywords
- DNA origami
- nanotechnology
- sequence design and optimization
- synthetic biology