Designing Rigid DNA Origami Templates for Molecular Visualization Using Cryo-EM

Ali Khoshouei; Georg Kempf; Volodymyr Mykhailiuk; Johanna Mariko Griessing; Maximilian Nicolas Honemann; Lukas Kater; Simone Cavadini; Hendrik Dietz

doi:10.1021/acs.nanolett.4c00915

Designing Rigid DNA Origami Templates for Molecular Visualization Using Cryo-EM

Ali Khoshouei, Georg Kempf, Volodymyr Mykhailiuk, Johanna Mariko Griessing, Maximilian Nicolas Honemann, Lukas Kater, Simone Cavadini, Hendrik Dietz

Chair of Biomolecular Nanotechnology

Research output: Contribution to journal › Article › peer-review

Abstract

DNA origami, a method for constructing nanostructures from DNA, offers potential for diverse scientific and technological applications due to its ability to integrate various molecular functionalities in a programmable manner. In this study, we examined the impact of internal crossover distribution and the compositional uniformity of staple strands on the structure of multilayer DNA origami using cryogenic electron microscopy (cryo-EM) single-particle analysis. A refined DNA object was utilized as an alignment framework in a host-guest model, where we successfully resolved an 8 kDa thrombin binding aptamer (TBA) linked to the host object. Our results broaden the spectrum of DNA in structural applications.

Original language	English
Journal	Nano Letters
DOIs	https://doi.org/10.1021/acs.nanolett.4c00915
State	Accepted/In press - 2024

Keywords

Cryo-EM
DNA origami
Design Optimization
Scaffolding
Thrombin Binding Aptamer

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10.1021/acs.nanolett.4c00915

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title = "Designing Rigid DNA Origami Templates for Molecular Visualization Using Cryo-EM",

abstract = "DNA origami, a method for constructing nanostructures from DNA, offers potential for diverse scientific and technological applications due to its ability to integrate various molecular functionalities in a programmable manner. In this study, we examined the impact of internal crossover distribution and the compositional uniformity of staple strands on the structure of multilayer DNA origami using cryogenic electron microscopy (cryo-EM) single-particle analysis. A refined DNA object was utilized as an alignment framework in a host-guest model, where we successfully resolved an 8 kDa thrombin binding aptamer (TBA) linked to the host object. Our results broaden the spectrum of DNA in structural applications.",

keywords = "Cryo-EM, DNA origami, Design Optimization, Scaffolding, Thrombin Binding Aptamer",

author = "Ali Khoshouei and Georg Kempf and Volodymyr Mykhailiuk and Griessing, {Johanna Mariko} and Honemann, {Maximilian Nicolas} and Lukas Kater and Simone Cavadini and Hendrik Dietz",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors. Published by American Chemical Society.",

year = "2024",

doi = "10.1021/acs.nanolett.4c00915",

language = "English",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

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T1 - Designing Rigid DNA Origami Templates for Molecular Visualization Using Cryo-EM

AU - Khoshouei, Ali

AU - Kempf, Georg

AU - Mykhailiuk, Volodymyr

AU - Griessing, Johanna Mariko

AU - Honemann, Maximilian Nicolas

AU - Kater, Lukas

AU - Cavadini, Simone

AU - Dietz, Hendrik

PY - 2024

Y1 - 2024

N2 - DNA origami, a method for constructing nanostructures from DNA, offers potential for diverse scientific and technological applications due to its ability to integrate various molecular functionalities in a programmable manner. In this study, we examined the impact of internal crossover distribution and the compositional uniformity of staple strands on the structure of multilayer DNA origami using cryogenic electron microscopy (cryo-EM) single-particle analysis. A refined DNA object was utilized as an alignment framework in a host-guest model, where we successfully resolved an 8 kDa thrombin binding aptamer (TBA) linked to the host object. Our results broaden the spectrum of DNA in structural applications.

AB - DNA origami, a method for constructing nanostructures from DNA, offers potential for diverse scientific and technological applications due to its ability to integrate various molecular functionalities in a programmable manner. In this study, we examined the impact of internal crossover distribution and the compositional uniformity of staple strands on the structure of multilayer DNA origami using cryogenic electron microscopy (cryo-EM) single-particle analysis. A refined DNA object was utilized as an alignment framework in a host-guest model, where we successfully resolved an 8 kDa thrombin binding aptamer (TBA) linked to the host object. Our results broaden the spectrum of DNA in structural applications.

KW - Cryo-EM

KW - DNA origami

KW - Design Optimization

KW - Scaffolding

KW - Thrombin Binding Aptamer

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U2 - 10.1021/acs.nanolett.4c00915

DO - 10.1021/acs.nanolett.4c00915

M3 - Article

AN - SCOPUS:85190168980

SN - 1530-6984

JO - Nano Letters

JF - Nano Letters

ER -

Designing Rigid DNA Origami Templates for Molecular Visualization Using Cryo-EM

Abstract

Keywords

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