Design and characterization of a rotating bed system bioreactor for tissue engineering applications

Fabienne Anton, Kirstin Suck, Solvig Diederichs, Larissa Behr, Bernd Hitzmann, Martijn Van Griensven, Thomas Scheper, Cornelia Kasper

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The main challenge in the development of bioreactors for tissue engineering is the delivery of a sufficient nutrient and oxygen supply for cell growth in a 3D environment. Thus, a new rotating bed system bioreactor for tissue engineering applications was developed. The system consists of a culture vessel as well as an integrated rotating bed of special porous ceramic discs and a process control unit connected with the reactor to ensure optimal culturing conditions. The aim of the project was the design and construction of a fully equipped rotating bed reactor, and in particular, the characterization and optimization of the system with regard to technical parameters such as mixing time and pH-control to guarantee optimal conditions for cell growth and differentiation. Furthermore, the applicability of the developed system was demonstrated by cultivation of osteoblast precursor cells. The porous structure of the ceramic discs and the external medium circulation loop provide an optimal environment for tissue generation in long-term cultivations. Mass transfer limitations were minimized by the slow rotation, which also provides the cells with sufficient nutrients and oxygen through alternate contact to air and medium. An osteoblast precursor cell line was successfully cultivated in this bioreactor for 28 days.

Original languageEnglish
Pages (from-to)140-147
Number of pages8
JournalBiotechnology Progress
Volume24
Issue number1
DOIs
StatePublished - Jan 2008
Externally publishedYes

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