TY - JOUR
T1 - Description of two cultivated and two uncultivated new Salinibacter species, one named following the rules of the bacteriological code
T2 - Salinibacter grassmerensis sp. nov.; and three named following the rules of the SeqCode: Salinibacter pepae sp. nov., Salinibacter abyssi sp. nov., and Salinibacter pampae sp. nov.
AU - Viver, Tomeu
AU - Conrad, Roth E.
AU - Lucio, Marianna
AU - Harir, Mourad
AU - Urdiain, Mercedes
AU - Gago, Juan F.
AU - Suárez-Suárez, Ana
AU - Bustos-Caparros, Esteban
AU - Sanchez-Martinez, Rodrigo
AU - Mayol, Eva
AU - Fassetta, Federico
AU - Pang, Jinfeng
AU - Mădălin Gridan, Ionuț
AU - Venter, Stephanus
AU - Santos, Fernando
AU - Baxter, Bonnie
AU - Llames, María E.
AU - Cristea, Adorján
AU - Banciu, Horia L.
AU - Hedlund, Brian P.
AU - Stott, Matthew B.
AU - Kämpfer, Peter
AU - Amann, Rudolf
AU - Schmitt-Kopplin, Philippe
AU - Konstantinidis, Konstantinos T.
AU - Rossello-Mora, Ramon
N1 - Publisher Copyright:
© 2023 The Author(s)
PY - 2023/5
Y1 - 2023/5
N2 - Current -omics methods allow the collection of a large amount of information that helps in describing the microbial diversity in nature. Here, and as a result of a culturomic approach that rendered the collection of thousands of isolates from 5 different hypersaline sites (in Spain, USA and New Zealand), we obtained 21 strains that represent two new Salinibacter species. For these species we propose the names Salinibacter pepae sp. nov. and Salinibacter grassmerensis sp. nov. (showing average nucleotide identity (ANI) values < 95.09% and 87.08% with Sal. ruber M31T, respectively). Metabolomics revealed species-specific discriminative profiles. Sal. ruber strains were distinguished by a higher percentage of polyunsaturated fatty acids and specific N-functionalized fatty acids; and Sal. altiplanensis was distinguished by an increased number of glycosylated molecules. Based on sequence characteristics and inferred phenotype of metagenome-assembled genomes (MAGs), we describe two new members of the genus Salinibacter. These species dominated in different sites and always coexisted with Sal. ruber and Sal. pepae. Based on the MAGs from three Argentinian lakes in the Pampa region of Argentina and the MAG of the Romanian lake Fără Fund, we describe the species Salinibacter pampae sp. nov. and Salinibacter abyssi sp. nov. respectively (showing ANI values 90.94% and 91.48% with Sal. ruber M31T, respectively). Sal. grassmerensis sp. nov. name was formed according to the rules of the International Code for Nomenclature of Prokaryotes (ICNP), and Sal. pepae, Sal. pampae sp. nov. and Sal. abyssi sp. nov. are proposed following the rules of the newly published Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode). This work constitutes an example on how classification under ICNP and SeqCode can coexist, and how the official naming a cultivated organism for which the deposit in public repositories is difficult finds an intermediate solution.
AB - Current -omics methods allow the collection of a large amount of information that helps in describing the microbial diversity in nature. Here, and as a result of a culturomic approach that rendered the collection of thousands of isolates from 5 different hypersaline sites (in Spain, USA and New Zealand), we obtained 21 strains that represent two new Salinibacter species. For these species we propose the names Salinibacter pepae sp. nov. and Salinibacter grassmerensis sp. nov. (showing average nucleotide identity (ANI) values < 95.09% and 87.08% with Sal. ruber M31T, respectively). Metabolomics revealed species-specific discriminative profiles. Sal. ruber strains were distinguished by a higher percentage of polyunsaturated fatty acids and specific N-functionalized fatty acids; and Sal. altiplanensis was distinguished by an increased number of glycosylated molecules. Based on sequence characteristics and inferred phenotype of metagenome-assembled genomes (MAGs), we describe two new members of the genus Salinibacter. These species dominated in different sites and always coexisted with Sal. ruber and Sal. pepae. Based on the MAGs from three Argentinian lakes in the Pampa region of Argentina and the MAG of the Romanian lake Fără Fund, we describe the species Salinibacter pampae sp. nov. and Salinibacter abyssi sp. nov. respectively (showing ANI values 90.94% and 91.48% with Sal. ruber M31T, respectively). Sal. grassmerensis sp. nov. name was formed according to the rules of the International Code for Nomenclature of Prokaryotes (ICNP), and Sal. pepae, Sal. pampae sp. nov. and Sal. abyssi sp. nov. are proposed following the rules of the newly published Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode). This work constitutes an example on how classification under ICNP and SeqCode can coexist, and how the official naming a cultivated organism for which the deposit in public repositories is difficult finds an intermediate solution.
KW - Genomics
KW - ICNP
KW - Phylogeny
KW - Salinibacter
KW - SeqCode
KW - Taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85150778460&partnerID=8YFLogxK
U2 - 10.1016/j.syapm.2023.126416
DO - 10.1016/j.syapm.2023.126416
M3 - Article
AN - SCOPUS:85150778460
SN - 0723-2020
VL - 46
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 3
M1 - 126416
ER -