Deletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress

Moritz Mühlhofer, Felix Offensperger, Sarah Reschke, Georg Wallmann, Gergely Csaba, Evi Berchtold, Maximilian Riedl, Helmut Blum, Martin Haslbeck, Ralf Zimmer, Johannes Buchner

Research output: Contribution to journalArticlepeer-review

Abstract

The response to proteotoxic stresses such as heat shock allows organisms to maintain protein homeostasis under changing environmental conditions. We asked what happens if an organism can no longer react to cytosolic proteotoxic stress. To test this, we deleted or depleted, either individually or in combination, the stress-responsive transcription factors Msn2, Msn4, and Hsf1 in Saccharomyces cerevisiae. Our study reveals a combination of survival strategies, which together protect essential proteins. Msn2 and 4 broadly reprogram transcription, triggering the response to oxidative stress, as well as biosynthesis of the protective sugar trehalose and glycolytic enzymes, while Hsf1 mainly induces the synthesis of molecular chaperones and reverses the transcriptional response upon prolonged mild heat stress (adaptation).

Original languageEnglish
Pages (from-to)635-657
Number of pages23
JournalFEBS Letters
Volume598
Issue number6
DOIs
StatePublished - Mar 2024

Keywords

  • Hsf1
  • Msn2/4
  • heat shock response
  • proteome
  • proteostasis
  • transcriptome

Fingerprint

Dive into the research topics of 'Deletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress'. Together they form a unique fingerprint.

Cite this