TY - JOUR
T1 - Defined conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin
AU - Höfner, Thomas
AU - Eisen, Christian
AU - Klein, Corinna
AU - Rigo-Watermeier, Teresa
AU - Goeppinger, Stephan M.
AU - Jauch, Anna
AU - Schoell, Brigitte
AU - Vogel, Vanessa
AU - Noll, Elisa
AU - Weichert, Wilko
AU - Baccelli, Irène
AU - Schillert, Anja
AU - Wagner, Steve
AU - Pahernik, Sascha
AU - Sprick, Martin R.
AU - Trumpp, Andreas
N1 - Publisher Copyright:
© 2015 The Authors.
PY - 2015/3/10
Y1 - 2015/3/10
N2 - Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin-SCA-1+CD49f+TROP2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin-CD49f+TROP2high PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin.
AB - Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin-SCA-1+CD49f+TROP2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin-CD49f+TROP2high PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin.
UR - http://www.scopus.com/inward/record.url?scp=84924633272&partnerID=8YFLogxK
U2 - 10.1016/j.stemcr.2015.01.015
DO - 10.1016/j.stemcr.2015.01.015
M3 - Article
C2 - 25702639
AN - SCOPUS:84924633272
SN - 2213-6711
VL - 4
SP - 503
EP - 518
JO - Stem Cell Reports
JF - Stem Cell Reports
IS - 3
ER -