Dark coffee consumption protects human blood cells from spontaneous DNA damage

Gudrun Pahlke; Eva Attakpah; Georg Aichinger; Katarina Ahlberg; Christina Maria Hochkogler; Kerstin Schweiger; Dorothea Schipp; Veronika Somoza; Doris Marko

doi:10.1016/j.jff.2019.02.008

Dark coffee consumption protects human blood cells from spontaneous DNA damage

Gudrun Pahlke, Eva Attakpah, Georg Aichinger, Katarina Ahlberg, Christina Maria Hochkogler, Kerstin Schweiger, Dorothea Schipp, Veronika Somoza, Doris Marko

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Coffee increasingly attracts notice with respect to beneficial health effects. Our objective was to investigate DNA protective effects of a special roast coffee blend of pure Arabica (Coffea arabica L.) in healthy volunteers (n = 96), following a prospective, randomized, controlled study with parallel design (coffee versus water). Potential modulation of Nrf2 signaling was evaluated by focusing on its two master regulators, Nrf2 and Keap1, as well as on Nrf2 translocation in the volunteers’ lymphocytes (PBLs). In this context a newly established fluorescence imaging method for Nrf2 translocation analysis in PBLs turned out as feasible and eligible tool applicable for future studies. After chronical coffee consumption (8 weeks) spontaneous DNA strand breaks were significantly lower in the coffee group compared to water control, suggesting a protective effect of the coffee blend. Nrf2 signaling was remotely affected, indicating that additional mechanisms of protection from DNA damage need to be considered.

Original language	English
Pages (from-to)	285-295
Number of pages	11
Journal	Journal of Functional Foods
Volume	55
DOIs	https://doi.org/10.1016/j.jff.2019.02.008
State	Published - Apr 2019
Externally published	Yes

Keywords

Coffee
DNA integrity
Keap1
Nrf2
human PBLs

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jff.2019.02.008

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title = "Dark coffee consumption protects human blood cells from spontaneous DNA damage",

abstract = "Coffee increasingly attracts notice with respect to beneficial health effects. Our objective was to investigate DNA protective effects of a special roast coffee blend of pure Arabica (Coffea arabica L.) in healthy volunteers (n = 96), following a prospective, randomized, controlled study with parallel design (coffee versus water). Potential modulation of Nrf2 signaling was evaluated by focusing on its two master regulators, Nrf2 and Keap1, as well as on Nrf2 translocation in the volunteers{\textquoteright} lymphocytes (PBLs). In this context a newly established fluorescence imaging method for Nrf2 translocation analysis in PBLs turned out as feasible and eligible tool applicable for future studies. After chronical coffee consumption (8 weeks) spontaneous DNA strand breaks were significantly lower in the coffee group compared to water control, suggesting a protective effect of the coffee blend. Nrf2 signaling was remotely affected, indicating that additional mechanisms of protection from DNA damage need to be considered.",

keywords = "Coffee, DNA integrity, Keap1, Nrf2, human PBLs",

author = "Gudrun Pahlke and Eva Attakpah and Georg Aichinger and Katarina Ahlberg and Hochkogler, {Christina Maria} and Kerstin Schweiger and Dorothea Schipp and Veronika Somoza and Doris Marko",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = apr,

doi = "10.1016/j.jff.2019.02.008",

language = "English",

volume = "55",

pages = "285--295",

journal = "Journal of Functional Foods",

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T1 - Dark coffee consumption protects human blood cells from spontaneous DNA damage

AU - Pahlke, Gudrun

AU - Attakpah, Eva

AU - Aichinger, Georg

AU - Ahlberg, Katarina

AU - Hochkogler, Christina Maria

AU - Schweiger, Kerstin

AU - Schipp, Dorothea

AU - Somoza, Veronika

AU - Marko, Doris

PY - 2019/4

Y1 - 2019/4

N2 - Coffee increasingly attracts notice with respect to beneficial health effects. Our objective was to investigate DNA protective effects of a special roast coffee blend of pure Arabica (Coffea arabica L.) in healthy volunteers (n = 96), following a prospective, randomized, controlled study with parallel design (coffee versus water). Potential modulation of Nrf2 signaling was evaluated by focusing on its two master regulators, Nrf2 and Keap1, as well as on Nrf2 translocation in the volunteers’ lymphocytes (PBLs). In this context a newly established fluorescence imaging method for Nrf2 translocation analysis in PBLs turned out as feasible and eligible tool applicable for future studies. After chronical coffee consumption (8 weeks) spontaneous DNA strand breaks were significantly lower in the coffee group compared to water control, suggesting a protective effect of the coffee blend. Nrf2 signaling was remotely affected, indicating that additional mechanisms of protection from DNA damage need to be considered.

AB - Coffee increasingly attracts notice with respect to beneficial health effects. Our objective was to investigate DNA protective effects of a special roast coffee blend of pure Arabica (Coffea arabica L.) in healthy volunteers (n = 96), following a prospective, randomized, controlled study with parallel design (coffee versus water). Potential modulation of Nrf2 signaling was evaluated by focusing on its two master regulators, Nrf2 and Keap1, as well as on Nrf2 translocation in the volunteers’ lymphocytes (PBLs). In this context a newly established fluorescence imaging method for Nrf2 translocation analysis in PBLs turned out as feasible and eligible tool applicable for future studies. After chronical coffee consumption (8 weeks) spontaneous DNA strand breaks were significantly lower in the coffee group compared to water control, suggesting a protective effect of the coffee blend. Nrf2 signaling was remotely affected, indicating that additional mechanisms of protection from DNA damage need to be considered.

KW - Coffee

KW - DNA integrity

KW - Keap1

KW - Nrf2

KW - human PBLs

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U2 - 10.1016/j.jff.2019.02.008

DO - 10.1016/j.jff.2019.02.008

M3 - Article

AN - SCOPUS:85062148008

SN - 1756-4646

VL - 55

SP - 285

EP - 295

JO - Journal of Functional Foods

JF - Journal of Functional Foods

ER -

Dark coffee consumption protects human blood cells from spontaneous DNA damage

Abstract

Keywords

UN SDGs

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