Corticotropin-releasing hormone regulates common target genes with divergent functions in corticotrope and neuronal cells

Cornelia Graf; Claudia Kuehne; Markus Panhuysen; Benno Puetz; Peter Weber; Florian Holsboer; Wolfgang Wurst; Jan M. Deussing

doi:10.1016/j.mce.2012.05.007

Corticotropin-releasing hormone regulates common target genes with divergent functions in corticotrope and neuronal cells

Cornelia Graf, Claudia Kuehne, Markus Panhuysen, Benno Puetz, Peter Weber, Florian Holsboer, Wolfgang Wurst, Jan M. Deussing

Chair of Developmental Genetics

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

As a key regulator of the neuroendocrine stress axis and as a neuromodulator in the brain, the neuropeptide corticotropin-releasing hormone (CRH) plays an important role in various diseases of the central nervous system. Its cognate receptor CRH receptor type 1 (CRHR1) is a potential novel target for the therapeutic intervention in major depressive disorder. Therefore, a more precise understanding of involved intracellular signaling mechanisms is essential. The objective of this project was to identify specific target genes of CRHR1-mediated signaling pathways in the corticotrope cell line AtT-20 and in the neuronal cell line HN9 using microarray technology and qRT-PCR, respectively. In addition, we assessed the capacity of validated target genes to directly impact on CRHR1-dependent signaling using reporter assays. Thereby, we identified a set of CRHR1 downstream targets with diverging and cell type-specific roles which strengthen the role of CRH and CRHR1 as dynamic modulators of a variety of signal transduction mechanisms and cellular processes.

Original language	English
Pages (from-to)	29-38
Number of pages	10
Journal	Molecular and Cellular Endocrinology
Volume	362
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mce.2012.05.007
State	Published - 15 Oct 2012

Keywords

AtT-20
CRHR1
Corticotropin-releasing hormone
Expression profiling
HPA axis

Access to Document

10.1016/j.mce.2012.05.007

Cite this

@article{5abcffee06434bdd97d00fd25246e81c,

title = "Corticotropin-releasing hormone regulates common target genes with divergent functions in corticotrope and neuronal cells",

abstract = "As a key regulator of the neuroendocrine stress axis and as a neuromodulator in the brain, the neuropeptide corticotropin-releasing hormone (CRH) plays an important role in various diseases of the central nervous system. Its cognate receptor CRH receptor type 1 (CRHR1) is a potential novel target for the therapeutic intervention in major depressive disorder. Therefore, a more precise understanding of involved intracellular signaling mechanisms is essential. The objective of this project was to identify specific target genes of CRHR1-mediated signaling pathways in the corticotrope cell line AtT-20 and in the neuronal cell line HN9 using microarray technology and qRT-PCR, respectively. In addition, we assessed the capacity of validated target genes to directly impact on CRHR1-dependent signaling using reporter assays. Thereby, we identified a set of CRHR1 downstream targets with diverging and cell type-specific roles which strengthen the role of CRH and CRHR1 as dynamic modulators of a variety of signal transduction mechanisms and cellular processes.",

keywords = "AtT-20, CRHR1, Corticotropin-releasing hormone, Expression profiling, HPA axis",

author = "Cornelia Graf and Claudia Kuehne and Markus Panhuysen and Benno Puetz and Peter Weber and Florian Holsboer and Wolfgang Wurst and Deussing, {Jan M.}",

note = "Funding Information: We thank Carola Hetzel and Katharine Webb for for editorial assistance nts on the manuscript. This work was partially supported by the Bundesministerium f{\"u}r Bildung und Forschung within the framework of the NGFN-Plus (FKZ: 01GS08151 and 01GS08155) and by the Initiative and Networking Fund of the Helmholtz Association in the framework of the Helmholtz Alliance for Mental Health in an Ageing Society (HA-215). ",

year = "2012",

month = oct,

day = "15",

doi = "10.1016/j.mce.2012.05.007",

language = "English",

volume = "362",

pages = "29--38",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

}

TY - JOUR

T1 - Corticotropin-releasing hormone regulates common target genes with divergent functions in corticotrope and neuronal cells

AU - Graf, Cornelia

AU - Kuehne, Claudia

AU - Panhuysen, Markus

AU - Puetz, Benno

AU - Weber, Peter

AU - Holsboer, Florian

AU - Wurst, Wolfgang

AU - Deussing, Jan M.

N1 - Funding Information: We thank Carola Hetzel and Katharine Webb for for editorial assistance nts on the manuscript. This work was partially supported by the Bundesministerium für Bildung und Forschung within the framework of the NGFN-Plus (FKZ: 01GS08151 and 01GS08155) and by the Initiative and Networking Fund of the Helmholtz Association in the framework of the Helmholtz Alliance for Mental Health in an Ageing Society (HA-215).

PY - 2012/10/15

Y1 - 2012/10/15

N2 - As a key regulator of the neuroendocrine stress axis and as a neuromodulator in the brain, the neuropeptide corticotropin-releasing hormone (CRH) plays an important role in various diseases of the central nervous system. Its cognate receptor CRH receptor type 1 (CRHR1) is a potential novel target for the therapeutic intervention in major depressive disorder. Therefore, a more precise understanding of involved intracellular signaling mechanisms is essential. The objective of this project was to identify specific target genes of CRHR1-mediated signaling pathways in the corticotrope cell line AtT-20 and in the neuronal cell line HN9 using microarray technology and qRT-PCR, respectively. In addition, we assessed the capacity of validated target genes to directly impact on CRHR1-dependent signaling using reporter assays. Thereby, we identified a set of CRHR1 downstream targets with diverging and cell type-specific roles which strengthen the role of CRH and CRHR1 as dynamic modulators of a variety of signal transduction mechanisms and cellular processes.

AB - As a key regulator of the neuroendocrine stress axis and as a neuromodulator in the brain, the neuropeptide corticotropin-releasing hormone (CRH) plays an important role in various diseases of the central nervous system. Its cognate receptor CRH receptor type 1 (CRHR1) is a potential novel target for the therapeutic intervention in major depressive disorder. Therefore, a more precise understanding of involved intracellular signaling mechanisms is essential. The objective of this project was to identify specific target genes of CRHR1-mediated signaling pathways in the corticotrope cell line AtT-20 and in the neuronal cell line HN9 using microarray technology and qRT-PCR, respectively. In addition, we assessed the capacity of validated target genes to directly impact on CRHR1-dependent signaling using reporter assays. Thereby, we identified a set of CRHR1 downstream targets with diverging and cell type-specific roles which strengthen the role of CRH and CRHR1 as dynamic modulators of a variety of signal transduction mechanisms and cellular processes.

KW - AtT-20

KW - CRHR1

KW - Corticotropin-releasing hormone

KW - Expression profiling

KW - HPA axis

UR - http://www.scopus.com/inward/record.url?scp=84865277719&partnerID=8YFLogxK

U2 - 10.1016/j.mce.2012.05.007

DO - 10.1016/j.mce.2012.05.007

M3 - Article

C2 - 22659651

AN - SCOPUS:84865277719

SN - 0303-7207

VL - 362

SP - 29

EP - 38

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

IS - 1-2

ER -

Corticotropin-releasing hormone regulates common target genes with divergent functions in corticotrope and neuronal cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this