Correlating whole brain neural activity with behavior in head-fixed larval Zebrafish

Michael B. Orger, Ruben Portugues

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

8 Scopus citations

Abstract

We present a protocol to combine behavioral recording and imaging using 2-photon laser-scanning microscopy in head-fixed larval zebrafish that express a genetically encoded calcium indicator. The steps involve restraining the larva in agarose, setting up optics that allow projection of a visual stimulus and infrared illumination to monitor behavior, and analysis of the neuronal and behavioral data.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages307-320
Number of pages14
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1451
ISSN (Print)1064-3745

Keywords

  • 2-Photon microscopy
  • Behavior
  • Whole-brain imaging
  • Zebrafish

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