Continuous Perfusion Experiments on 3D Cell Proliferation in Acoustic Levitation

Luca Fabiano, Shilpi Pandey, Martin Brischwein, Morteza Hasanzadeh Kafshgari, Oliver Hayden

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


An acoustofluidic trap is used for accurate 3D cell proliferation and cell function analysis in levitation. The prototype trap can be integrated with any microscope setup, allowing continuous perfusion experiments with temperature and flow control under optical inspection. To describe the trap function, we present a mathematical and FEM-based COMSOL model for the acoustic mode that defines the nodal position of trapped objects in the spherical cavity aligned with the microscope field of view and depth of field. Continuous perfusion experiments were conducted in sterile conditions over 55 h with a K562 cell line, allowing for deterministic monitoring. The acoustofluidic platform allows for rational in vitro cell testing imitating in vivo conditions such as cell function tests or cell–cell interactions.

Original languageEnglish
Article number436
Issue number4
StatePublished - Apr 2024


  • 3D cell trap
  • bulk acoustics
  • in vitro cell test
  • microfluidics


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