Conditional gene targeting: A refined method for genetic studies in neurosciences

Jane Beil; Isabelle M. Mansuy; Thorsten Buch

doi:10.1007/978-1-62703-517-0_1

Conditional gene targeting: A refined method for genetic studies in neurosciences

Jane Beil
, Isabelle M. Mansuy
, Thorsten Buch

Default

Technical University of Munich
University of Zurich

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Conditional mutagenesis in mice is a key approach in neurosciences that makes it possible to investigate the functions of defined genes within certain neural subpopulations. The approach is based on the combination of transgenic expression of a recombinase with targeted candidate genes containing recognition sequences of this recombinase generated by gene targeting. Here we describe three major recombination systems, the Cre/loxP, Flp/FRT, and Dre/rox, and present a procedure that allows the generation of target alleles for these systems. We provide detailed protocols for the culture and transfection of embryonic stem (ES) cells and for the identification and confirmation of homologous recombinants. This chapter also provides an overview of recent developments such as gene targeting in oocytes and discusses future perspectives.

Original language	English
Title of host publication	Multidisciplinary Tools for Investigating Synaptic Plasticity
Publisher	Humana Press Inc.
Pages	3-41
Number of pages	39
ISBN (Print)	9781627035163
DOIs	https://doi.org/10.1007/978-1-62703-517-0_1
State	Published - 2013

Publication series

Name	Neuromethods
Volume	81
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Conditional
Cre/loxP
ES cells
Flp/FRT
Gene targeting
Mutant mice
Recombination
Targeting vector

Access to Document

10.1007/978-1-62703-517-0_1

Cite this

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abstract = "Conditional mutagenesis in mice is a key approach in neurosciences that makes it possible to investigate the functions of defined genes within certain neural subpopulations. The approach is based on the combination of transgenic expression of a recombinase with targeted candidate genes containing recognition sequences of this recombinase generated by gene targeting. Here we describe three major recombination systems, the Cre/loxP, Flp/FRT, and Dre/rox, and present a procedure that allows the generation of target alleles for these systems. We provide detailed protocols for the culture and transfection of embryonic stem (ES) cells and for the identification and confirmation of homologous recombinants. This chapter also provides an overview of recent developments such as gene targeting in oocytes and discusses future perspectives.",

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T2 - A refined method for genetic studies in neurosciences

AU - Beil, Jane

AU - Mansuy, Isabelle M.

AU - Buch, Thorsten

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AB - Conditional mutagenesis in mice is a key approach in neurosciences that makes it possible to investigate the functions of defined genes within certain neural subpopulations. The approach is based on the combination of transgenic expression of a recombinase with targeted candidate genes containing recognition sequences of this recombinase generated by gene targeting. Here we describe three major recombination systems, the Cre/loxP, Flp/FRT, and Dre/rox, and present a procedure that allows the generation of target alleles for these systems. We provide detailed protocols for the culture and transfection of embryonic stem (ES) cells and for the identification and confirmation of homologous recombinants. This chapter also provides an overview of recent developments such as gene targeting in oocytes and discusses future perspectives.

KW - Conditional

KW - Cre/loxP

KW - ES cells

KW - Flp/FRT

KW - Gene targeting

KW - Mutant mice

KW - Recombination

KW - Targeting vector

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AN - SCOPUS:84880495254

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T3 - Neuromethods

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BT - Multidisciplinary Tools for Investigating Synaptic Plasticity

PB - Humana Press Inc.

ER -

Conditional gene targeting: A refined method for genetic studies in neurosciences

Abstract

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UN SDGs

Keywords

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