TY - JOUR
T1 - Computational solvent system screening for the separation of tocopherols with centrifugal partition chromatography using deep eutectic solvent-based biphasic systems
AU - Bezold, Franziska
AU - Weinberger, Maria E.
AU - Minceva, Mirjana
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/3/31
Y1 - 2017/3/31
N2 - Tocopherols are a class of molecules with vitamin E activity. Among those, α-tocopherol is the most important vitamin E source in the human diet. The purification of tocopherols involving biphasic liquid systems can be challenging since these vitamins are poorly soluble in water. Deep eutectic solvents (DES) can be used to form water-free biphasic systems and have already proven applicable for centrifugal partition chromatography separations. In this work, a computational solvent system screening was performed using the predictive thermodynamic model COSMO-RS. Liquid-liquid equilibria of solvent systems composed of alkanes, alcohols and DES, as well as partition coefficients of α-tocopherol, β-tocopherol, γ-tocopherol, and σ-tocopherol in these biphasic solvent systems were calculated. From the results the best suited biphasic solvent system, namely heptane/ethanol/choline chloride-1,4-butanediol, was chosen and a batch injection of a tocopherol mixture, mainly consisting of α- and γ-tocopherol, was performed using a centrifugal partition chromatography set up (SCPE 250-BIO). A separation factor of 1.74 was achieved for α- and γ-tocopherol.
AB - Tocopherols are a class of molecules with vitamin E activity. Among those, α-tocopherol is the most important vitamin E source in the human diet. The purification of tocopherols involving biphasic liquid systems can be challenging since these vitamins are poorly soluble in water. Deep eutectic solvents (DES) can be used to form water-free biphasic systems and have already proven applicable for centrifugal partition chromatography separations. In this work, a computational solvent system screening was performed using the predictive thermodynamic model COSMO-RS. Liquid-liquid equilibria of solvent systems composed of alkanes, alcohols and DES, as well as partition coefficients of α-tocopherol, β-tocopherol, γ-tocopherol, and σ-tocopherol in these biphasic solvent systems were calculated. From the results the best suited biphasic solvent system, namely heptane/ethanol/choline chloride-1,4-butanediol, was chosen and a batch injection of a tocopherol mixture, mainly consisting of α- and γ-tocopherol, was performed using a centrifugal partition chromatography set up (SCPE 250-BIO). A separation factor of 1.74 was achieved for α- and γ-tocopherol.
KW - Biphasic solvent system screening
KW - COSMO-RS
KW - Centrifugal partition chromatography
KW - Deep eutectic solvents
KW - Tocopherols
KW - Vitamin E
UR - http://www.scopus.com/inward/record.url?scp=85014304396&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2017.02.059
DO - 10.1016/j.chroma.2017.02.059
M3 - Article
C2 - 28262315
AN - SCOPUS:85014304396
SN - 0021-9673
VL - 1491
SP - 153
EP - 158
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -