TY - JOUR
T1 - Comprehensive proteome analysis in Cenococcum geophilum Fr. as a tool to discover drought-related proteins
AU - Kerner, René
AU - Delgado-Eckert, Edgar
AU - Del Castillo, Estela
AU - Müller-Starck, Gerhard
AU - Peter, Martina
AU - Kuster, Bernhard
AU - Tisserant, Emilie
AU - Pritsch, Karin
N1 - Funding Information:
The authors would like to thank Sarah Sturm for excellent technical assistance and Frank Gaupels for providing chemical reagents. E. del Castillo gratefully acknowledges the Alexander von Humboldt Foundation, Germany, for support. This research was carried out with financial support from the Deutsche Forschungsgemeinschaft (SFB 607) and the Network of Excellence “EVOLTREE”.
PY - 2012/6/27
Y1 - 2012/6/27
N2 - Cenococcum geophilum is a widely distributed ectomycorrhizal fungus potentially playing a significant role in resistance and resilience mechanisms of its tree hosts exposed to drought stress. In this study, we performed a large scale protein analysis in pure cultures of C. geophilum in order to gain first global insights into the proteome assembly of this fungus. Using 1-D gel electrophoresis coupled with ESI-MS/MS, we indentified 638 unique proteins. Most of these proteins were related to the metabolic and cellular processes, and the transport machinery of cells. In a second step, we examined the influence of water deprivation on the proteome of C. geophilum pure cultures at three time points of gradually imposed drought. The results indicated that 12 proteins were differentially abundant in mycelia subjected to drought compared to controls. The induced responses in C. geophilum point towards regulation of osmotic stress, maintainance of cell integrity, and counteracting increased levels of reactive oxygen species formed during water deprivation.
AB - Cenococcum geophilum is a widely distributed ectomycorrhizal fungus potentially playing a significant role in resistance and resilience mechanisms of its tree hosts exposed to drought stress. In this study, we performed a large scale protein analysis in pure cultures of C. geophilum in order to gain first global insights into the proteome assembly of this fungus. Using 1-D gel electrophoresis coupled with ESI-MS/MS, we indentified 638 unique proteins. Most of these proteins were related to the metabolic and cellular processes, and the transport machinery of cells. In a second step, we examined the influence of water deprivation on the proteome of C. geophilum pure cultures at three time points of gradually imposed drought. The results indicated that 12 proteins were differentially abundant in mycelia subjected to drought compared to controls. The induced responses in C. geophilum point towards regulation of osmotic stress, maintainance of cell integrity, and counteracting increased levels of reactive oxygen species formed during water deprivation.
KW - Abiotic stress
KW - Cenococcum geophilum
KW - Drought
KW - Fungal proteomics
UR - https://www.scopus.com/pages/publications/84862222448
U2 - 10.1016/j.jprot.2012.04.039
DO - 10.1016/j.jprot.2012.04.039
M3 - Article
C2 - 22579754
AN - SCOPUS:84862222448
SN - 1874-3919
VL - 75
SP - 3707
EP - 3719
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - 12
ER -