Complete nucleotide sequence of the lipase gene from Staphylococcus hyicus cloned in Staphylococcus carnosus

Friedrich Götz, Fritz Popp, Edda Kom, Karl Heinz Schleifer

Research output: Contribution to journalArticlepeer-review

110 Scopus citations

Abstract

The lipase gene from Staphylococcus hyicus subsp. hyicus was cloned in Staphylococcus carnosus and Escherichia coli. In both host organisms the lipase gene is expressed and the enzyme is released to the medium. The cloned DNA insert is 2.5 kb in length and DNA sequencing has revealed the location of the gene, the ribosomal binding site and the presence of a typical signal sequence. The open reading frame comprises 1923 nucleotides and gives a preprotein of 641 amino acids with a predicted Mr of 71.382. At the 3′ end of the structural gene there are three consecutive stop codons and there is also a transcriptional termination signal.

Original languageEnglish
Pages (from-to)5895-5906
Number of pages12
JournalNucleic Acids Research
Volume13
Issue number16
DOIs
StatePublished - 26 Aug 1985
Externally publishedYes

Fingerprint

Dive into the research topics of 'Complete nucleotide sequence of the lipase gene from Staphylococcus hyicus cloned in Staphylococcus carnosus'. Together they form a unique fingerprint.

Cite this