TY - JOUR
T1 - Comparison of twin-cell centrifugal partition chromatographic columns with different cell volume
AU - Goll, Johannes
AU - Audo, Gregoire
AU - Minceva, Mirjana
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/8/7
Y1 - 2015/8/7
N2 - Two twin-cell centrifugal partition chromatographic columns (SCPC 250 and SCPE-250-BIO, Armen Instrument, France) with the same column volume but different cell size and number were compared in terms of stationary phase retention and column efficiency. The columns were tested with two types of solvent systems: a commonly used organic solvent based biphasic system from the ARIZONA solvent system family and a polymer/salt based aqueous two phase system (ATPS). The efficiency of the columns was evaluated by pulse injection experiments of two benzenediols (pyrocatechol and hydroquinone) in the case of the ARIZONA system and a protein mixture (myoglobin and lysozyme) in the case of the ATPS. As result of high stationary phase retention, the column with the lower number of larger twin-cells (SCPE-250-BIO) is suitable for protein separations using ATPS. On the other hand, due to higher column efficiency, the column with the greater number of smaller cells (SCPC 250) is superior for batch elution separations performed with standard liquid-liquid chromatography organic solvent based biphasic systems.
AB - Two twin-cell centrifugal partition chromatographic columns (SCPC 250 and SCPE-250-BIO, Armen Instrument, France) with the same column volume but different cell size and number were compared in terms of stationary phase retention and column efficiency. The columns were tested with two types of solvent systems: a commonly used organic solvent based biphasic system from the ARIZONA solvent system family and a polymer/salt based aqueous two phase system (ATPS). The efficiency of the columns was evaluated by pulse injection experiments of two benzenediols (pyrocatechol and hydroquinone) in the case of the ARIZONA system and a protein mixture (myoglobin and lysozyme) in the case of the ATPS. As result of high stationary phase retention, the column with the lower number of larger twin-cells (SCPE-250-BIO) is suitable for protein separations using ATPS. On the other hand, due to higher column efficiency, the column with the greater number of smaller cells (SCPC 250) is superior for batch elution separations performed with standard liquid-liquid chromatography organic solvent based biphasic systems.
KW - Aqueous two phase systems
KW - Centrifugal partition chromatography
KW - Column efficiency
KW - Countercurrent chromatography
KW - Protein separation
KW - Stationary phase retention
KW - Twin-cell hydrostatic column
UR - http://www.scopus.com/inward/record.url?scp=84937630611&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2015.05.077
DO - 10.1016/j.chroma.2015.05.077
M3 - Article
C2 - 26105780
AN - SCOPUS:84937630611
SN - 0021-9673
VL - 1406
SP - 129
EP - 135
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -