Comparison of the QCM and the SPR method for surface studies and immunological applications

C. Kößlinger; E. Uttenthaler; S. Drost; F. Aberl; H. Wolf; G. Brink; A. Stanglmaier; E. Sackmann

doi:10.1016/0925-4005(95)85023-6

Comparison of the QCM and the SPR method for surface studies and immunological applications

C. Kößlinger, E. Uttenthaler, S. Drost, F. Aberl, H. Wolf, G. Brink, A. Stanglmaier, E. Sackmann

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Abstract

A comparison between the quartz-crystal microbalance (QCM) and surface plasmon resonance (SPR) using an especially designed flow-through cell for simultaneous measurements and flow-injection analysis (FIA) is presented. We compare the effects due to unspecific binding of bovine serum albumin (BSA) to gold surfaces, specific binding between BSA and monoclonal antibodies, regeneration experiments, serum experiments with HIV antigens and the influence of the bulk properties of liquid samples. The basic theory and its consequences on measurement signals and experimental methods are discussed. The fundamental problem of the comparability of different measurement principles is considered. We suggest guide parameters, which allow an objective judgement of the performance. We do not find any advantage of one particular system concerning sensitivity and cross sensitivity.

Original language	English
Pages (from-to)	107-112
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	24
Issue number	1-3
DOIs	https://doi.org/10.1016/0925-4005(95)85023-6
State	Published - Mar 1995

Keywords

Immunology
Quartz-crystal microbalance
Surface plasmon resonance
Surface studies

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10.1016/0925-4005(95)85023-6

Cite this

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abstract = "A comparison between the quartz-crystal microbalance (QCM) and surface plasmon resonance (SPR) using an especially designed flow-through cell for simultaneous measurements and flow-injection analysis (FIA) is presented. We compare the effects due to unspecific binding of bovine serum albumin (BSA) to gold surfaces, specific binding between BSA and monoclonal antibodies, regeneration experiments, serum experiments with HIV antigens and the influence of the bulk properties of liquid samples. The basic theory and its consequences on measurement signals and experimental methods are discussed. The fundamental problem of the comparability of different measurement principles is considered. We suggest guide parameters, which allow an objective judgement of the performance. We do not find any advantage of one particular system concerning sensitivity and cross sensitivity.",

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T1 - Comparison of the QCM and the SPR method for surface studies and immunological applications

AU - Kößlinger, C.

AU - Uttenthaler, E.

AU - Drost, S.

AU - Aberl, F.

AU - Wolf, H.

AU - Brink, G.

AU - Stanglmaier, A.

AU - Sackmann, E.

PY - 1995/3

Y1 - 1995/3

N2 - A comparison between the quartz-crystal microbalance (QCM) and surface plasmon resonance (SPR) using an especially designed flow-through cell for simultaneous measurements and flow-injection analysis (FIA) is presented. We compare the effects due to unspecific binding of bovine serum albumin (BSA) to gold surfaces, specific binding between BSA and monoclonal antibodies, regeneration experiments, serum experiments with HIV antigens and the influence of the bulk properties of liquid samples. The basic theory and its consequences on measurement signals and experimental methods are discussed. The fundamental problem of the comparability of different measurement principles is considered. We suggest guide parameters, which allow an objective judgement of the performance. We do not find any advantage of one particular system concerning sensitivity and cross sensitivity.

AB - A comparison between the quartz-crystal microbalance (QCM) and surface plasmon resonance (SPR) using an especially designed flow-through cell for simultaneous measurements and flow-injection analysis (FIA) is presented. We compare the effects due to unspecific binding of bovine serum albumin (BSA) to gold surfaces, specific binding between BSA and monoclonal antibodies, regeneration experiments, serum experiments with HIV antigens and the influence of the bulk properties of liquid samples. The basic theory and its consequences on measurement signals and experimental methods are discussed. The fundamental problem of the comparability of different measurement principles is considered. We suggest guide parameters, which allow an objective judgement of the performance. We do not find any advantage of one particular system concerning sensitivity and cross sensitivity.

KW - Immunology

KW - Quartz-crystal microbalance

KW - Surface plasmon resonance

KW - Surface studies

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Comparison of the QCM and the SPR method for surface studies and immunological applications

Abstract

Keywords

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