Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing

Melanie Spornraft; Benedikt Kirchner; Michael W. Pfaffl; Irmgard Riedmaier

doi:10.1007/s10529-015-1788-2

Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing

Melanie Spornraft, Benedikt Kirchner, Michael W. Pfaffl, Irmgard Riedmaier

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Technical University of Munich

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17 Scopus citations

Abstract

Objectives: The small RNAs of bovine plasma and whole blood were analysed using next-generation sequencing to quantify, profile and compare the microRNAs (miRNA) and piRNA signatures in both bio fluids. Results: Evaluating read-count data resulted in a proportion of 5.0 ± 2.9 % of miRNAs in plasma while 38.2 ± 3.4 % were identified in whole blood. Regarding piRNAs, the percentages in both matrices were nearly the same: 1.4 ± 0.8 % of piRNAs in plasma and 1.9 ± 0.8 % in whole blood. Investigation of the ten most abundant miRNAs and piRNAs in both bio fluids revealed that two miRNAs and seven piRNAs were identical. Comparing the read-count values of these matching pairs highlighted that miRNA and piRNA levels in blood exceeded the abundance of their corresponding miRNAs and piRNAs in plasma, except liver-specific miR-122 and three piRNAs. Conclusions: The data strengthened evidence that the circulating small RNA signature in plasma is not only influenced by hematocytes and certain small RNAs could originate from other sources than cellular blood components.

Original language	English
Pages (from-to)	1165-1176
Number of pages	12
Journal	Biotechnology Letters
Volume	37
Issue number	6
DOIs	https://doi.org/10.1007/s10529-015-1788-2
State	Published - 16 Jun 2015

Keywords

Blood analysis
Next-generation sequencing (NGS)
Plasma analysis
Small RNA-Seq
Small RNAs
microRNA
piRNA

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10.1007/s10529-015-1788-2

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title = "Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing",

abstract = "Objectives: The small RNAs of bovine plasma and whole blood were analysed using next-generation sequencing to quantify, profile and compare the microRNAs (miRNA) and piRNA signatures in both bio fluids. Results: Evaluating read-count data resulted in a proportion of 5.0 ± 2.9 % of miRNAs in plasma while 38.2 ± 3.4 % were identified in whole blood. Regarding piRNAs, the percentages in both matrices were nearly the same: 1.4 ± 0.8 % of piRNAs in plasma and 1.9 ± 0.8 % in whole blood. Investigation of the ten most abundant miRNAs and piRNAs in both bio fluids revealed that two miRNAs and seven piRNAs were identical. Comparing the read-count values of these matching pairs highlighted that miRNA and piRNA levels in blood exceeded the abundance of their corresponding miRNAs and piRNAs in plasma, except liver-specific miR-122 and three piRNAs. Conclusions: The data strengthened evidence that the circulating small RNA signature in plasma is not only influenced by hematocytes and certain small RNAs could originate from other sources than cellular blood components.",

keywords = "Blood analysis, Next-generation sequencing (NGS), Plasma analysis, Small RNA-Seq, Small RNAs, microRNA, piRNA",

author = "Melanie Spornraft and Benedikt Kirchner and Pfaffl, {Michael W.} and Irmgard Riedmaier",

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doi = "10.1007/s10529-015-1788-2",

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AU - Spornraft, Melanie

AU - Kirchner, Benedikt

AU - Pfaffl, Michael W.

AU - Riedmaier, Irmgard

PY - 2015/6/16

Y1 - 2015/6/16

N2 - Objectives: The small RNAs of bovine plasma and whole blood were analysed using next-generation sequencing to quantify, profile and compare the microRNAs (miRNA) and piRNA signatures in both bio fluids. Results: Evaluating read-count data resulted in a proportion of 5.0 ± 2.9 % of miRNAs in plasma while 38.2 ± 3.4 % were identified in whole blood. Regarding piRNAs, the percentages in both matrices were nearly the same: 1.4 ± 0.8 % of piRNAs in plasma and 1.9 ± 0.8 % in whole blood. Investigation of the ten most abundant miRNAs and piRNAs in both bio fluids revealed that two miRNAs and seven piRNAs were identical. Comparing the read-count values of these matching pairs highlighted that miRNA and piRNA levels in blood exceeded the abundance of their corresponding miRNAs and piRNAs in plasma, except liver-specific miR-122 and three piRNAs. Conclusions: The data strengthened evidence that the circulating small RNA signature in plasma is not only influenced by hematocytes and certain small RNAs could originate from other sources than cellular blood components.

AB - Objectives: The small RNAs of bovine plasma and whole blood were analysed using next-generation sequencing to quantify, profile and compare the microRNAs (miRNA) and piRNA signatures in both bio fluids. Results: Evaluating read-count data resulted in a proportion of 5.0 ± 2.9 % of miRNAs in plasma while 38.2 ± 3.4 % were identified in whole blood. Regarding piRNAs, the percentages in both matrices were nearly the same: 1.4 ± 0.8 % of piRNAs in plasma and 1.9 ± 0.8 % in whole blood. Investigation of the ten most abundant miRNAs and piRNAs in both bio fluids revealed that two miRNAs and seven piRNAs were identical. Comparing the read-count values of these matching pairs highlighted that miRNA and piRNA levels in blood exceeded the abundance of their corresponding miRNAs and piRNAs in plasma, except liver-specific miR-122 and three piRNAs. Conclusions: The data strengthened evidence that the circulating small RNA signature in plasma is not only influenced by hematocytes and certain small RNAs could originate from other sources than cellular blood components.

KW - Blood analysis

KW - Next-generation sequencing (NGS)

KW - Plasma analysis

KW - Small RNA-Seq

KW - Small RNAs

KW - microRNA

KW - piRNA

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JF - Biotechnology Letters

IS - 6

ER -

Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing

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Keywords

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