Comparison of the Enzymatic Activities of Dehydroascorbic acid Reductase, Glutathione Reductase, Catalase, Peroxidase and Superoxide Dismutase of Healthy and Damaged Spruce Needles (Picea abies (L.) Karst.)'

Wolfgang F. Osswaldi; Robert Kraus; Susanne Hippeli; Beatrice Benz; Regina Volpert; Erich F. Elstner

doi:10.1016/S0176-1617(11)81721-9

Comparison of the Enzymatic Activities of Dehydroascorbic acid Reductase, Glutathione Reductase, Catalase, Peroxidase and Superoxide Dismutase of Healthy and Damaged Spruce Needles (Picea abies (L.) Karst.)'

Wolfgang F. Osswaldi, Robert Kraus, Susanne Hippeli, Beatrice Benz, Regina Volpert, Erich F. Elstner

Life Sciences

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

The stability behaviour and the recovery rates of spruce needle enzymes such as superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were determined. Also the isoelectric points were measured for catalase to be 8.0, for peroxidase to be 6.0 and 9.4 and for glutathione reductase to be 4.6. Furthermore, the activities of superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were compared for healthy and damaged spruce needles. A significant increase in enzymatic activities was measured for dehydroascorbic acid reductase glutathione reductase and catalase of damaged compared to healthy needle segments. However, no difference in enzymatic activities was found for superoxide dismutase and peroxidase of healthy and damaged needle samples.

Original language	English
Pages (from-to)	742-748
Number of pages	7
Journal	Journal of Plant Physiology
Volume	139
Issue number	6
DOIs	https://doi.org/10.1016/S0176-1617(11)81721-9
State	Published - 1992

Keywords

Cat
DHARed
EDTA
GR
GSH
GSSG
NADPH
POD
PVP
SOD
Spruce decline
a-NED
catalase
ctNaphtylethylenediamin
dehydroascorbic acid reductase
ethylenediamine tetraacetic acid
glutathione (oxidized form)
glutathione (reduced form)
glutathione reductase
hydrogen peroxide scavenging enzymes
isoelectric points
nicotinamideadeninedinucleotide phosphate (reduced form)
peroxidase
pigment bleaching
polyvinylpyrrolidone
superoxide dismutase

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Osswaldi, W. F., Kraus, R., Hippeli, S., Benz, B., Volpert, R., & Elstner, E. F. (1992). Comparison of the Enzymatic Activities of Dehydroascorbic acid Reductase, Glutathione Reductase, Catalase, Peroxidase and Superoxide Dismutase of Healthy and Damaged Spruce Needles (Picea abies (L.) Karst.)'. Journal of Plant Physiology, 139(6), 742-748. https://doi.org/10.1016/S0176-1617(11)81721-9

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title = "Comparison of the Enzymatic Activities of Dehydroascorbic acid Reductase, Glutathione Reductase, Catalase, Peroxidase and Superoxide Dismutase of Healthy and Damaged Spruce Needles (Picea abies (L.) Karst.)'",

abstract = "The stability behaviour and the recovery rates of spruce needle enzymes such as superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were determined. Also the isoelectric points were measured for catalase to be 8.0, for peroxidase to be 6.0 and 9.4 and for glutathione reductase to be 4.6. Furthermore, the activities of superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were compared for healthy and damaged spruce needles. A significant increase in enzymatic activities was measured for dehydroascorbic acid reductase glutathione reductase and catalase of damaged compared to healthy needle segments. However, no difference in enzymatic activities was found for superoxide dismutase and peroxidase of healthy and damaged needle samples.",

keywords = "Cat, DHARed, EDTA, GR, GSH, GSSG, NADPH, POD, PVP, SOD, Spruce decline, a-NED, catalase, ctNaphtylethylenediamin, dehydroascorbic acid reductase, ethylenediamine tetraacetic acid, glutathione (oxidized form), glutathione (reduced form), glutathione reductase, hydrogen peroxide scavenging enzymes, isoelectric points, nicotinamideadeninedinucleotide phosphate (reduced form), peroxidase, pigment bleaching, polyvinylpyrrolidone, superoxide dismutase",

author = "Osswaldi, {Wolfgang F.} and Robert Kraus and Susanne Hippeli and Beatrice Benz and Regina Volpert and Elstner, {Erich F.}",

year = "1992",

doi = "10.1016/S0176-1617(11)81721-9",

language = "English",

volume = "139",

pages = "742--748",

journal = "Journal of Plant Physiology",

issn = "0176-1617",

publisher = "Elsevier GmbH",

number = "6",

}

Osswaldi, WF, Kraus, R, Hippeli, S, Benz, B, Volpert, R & Elstner, EF 1992, 'Comparison of the Enzymatic Activities of Dehydroascorbic acid Reductase, Glutathione Reductase, Catalase, Peroxidase and Superoxide Dismutase of Healthy and Damaged Spruce Needles (Picea abies (L.) Karst.)'', Journal of Plant Physiology, vol. 139, no. 6, pp. 742-748. https://doi.org/10.1016/S0176-1617(11)81721-9

Comparison of the Enzymatic Activities of Dehydroascorbic acid Reductase, Glutathione Reductase, Catalase, Peroxidase and Superoxide Dismutase of Healthy and Damaged Spruce Needles (Picea abies (L.) Karst.)'. / Osswaldi, Wolfgang F.; Kraus, Robert; Hippeli, Susanne et al.
In: Journal of Plant Physiology, Vol. 139, No. 6, 1992, p. 742-748.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Comparison of the Enzymatic Activities of Dehydroascorbic acid Reductase, Glutathione Reductase, Catalase, Peroxidase and Superoxide Dismutase of Healthy and Damaged Spruce Needles (Picea abies (L.) Karst.)'

AU - Osswaldi, Wolfgang F.

AU - Kraus, Robert

AU - Hippeli, Susanne

AU - Benz, Beatrice

AU - Volpert, Regina

AU - Elstner, Erich F.

PY - 1992

Y1 - 1992

N2 - The stability behaviour and the recovery rates of spruce needle enzymes such as superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were determined. Also the isoelectric points were measured for catalase to be 8.0, for peroxidase to be 6.0 and 9.4 and for glutathione reductase to be 4.6. Furthermore, the activities of superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were compared for healthy and damaged spruce needles. A significant increase in enzymatic activities was measured for dehydroascorbic acid reductase glutathione reductase and catalase of damaged compared to healthy needle segments. However, no difference in enzymatic activities was found for superoxide dismutase and peroxidase of healthy and damaged needle samples.

AB - The stability behaviour and the recovery rates of spruce needle enzymes such as superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were determined. Also the isoelectric points were measured for catalase to be 8.0, for peroxidase to be 6.0 and 9.4 and for glutathione reductase to be 4.6. Furthermore, the activities of superoxide dismutase, dehydroascorbic acid reductase, glutathione reductase, catalase and peroxidase were compared for healthy and damaged spruce needles. A significant increase in enzymatic activities was measured for dehydroascorbic acid reductase glutathione reductase and catalase of damaged compared to healthy needle segments. However, no difference in enzymatic activities was found for superoxide dismutase and peroxidase of healthy and damaged needle samples.

KW - Cat

KW - DHARed

KW - EDTA

KW - GR

KW - GSH

KW - GSSG

KW - NADPH

KW - POD

KW - PVP

KW - SOD

KW - Spruce decline

KW - a-NED

KW - catalase

KW - ctNaphtylethylenediamin

KW - dehydroascorbic acid reductase

KW - ethylenediamine tetraacetic acid

KW - glutathione (oxidized form)

KW - glutathione (reduced form)

KW - glutathione reductase

KW - hydrogen peroxide scavenging enzymes

KW - isoelectric points

KW - nicotinamideadeninedinucleotide phosphate (reduced form)

KW - peroxidase

KW - pigment bleaching

KW - polyvinylpyrrolidone

KW - superoxide dismutase

UR - http://www.scopus.com/inward/record.url?scp=85025528184&partnerID=8YFLogxK

U2 - 10.1016/S0176-1617(11)81721-9

DO - 10.1016/S0176-1617(11)81721-9

M3 - Article

AN - SCOPUS:85025528184

SN - 0176-1617

VL - 139

SP - 742

EP - 748

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

IS - 6

ER -

Comparison of the Enzymatic Activities of Dehydroascorbic acid Reductase, Glutathione Reductase, Catalase, Peroxidase and Superoxide Dismutase of Healthy and Damaged Spruce Needles (Picea abies (L.) Karst.)'

Abstract

Keywords

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