TY - JOUR
T1 - Comparison of liquid chromatography-mass spectrometry interfaces for the analysis of polar metabolites of benzo[a]pyrene
AU - Koeber, Robert
AU - Niessner, Reinhard
AU - Bayona, Josep Maria
PY - 1997
Y1 - 1997
N2 - The performance of two liquid chromatography-mass spectrometry (LC/MS) interfacing techniques, thermospray (TSP) and atmospheric pressure chemical ionization (APCI), for the analysis of benzo[a]pyrene (BaP) metabolites (hydroxy, epoxy and quinone derivatives) was compared. Interface and detection parameters such as source temperature, eluent composition or flow rate were optimized using negative ion mode. In TSP, the main ions are mostly [M]-, [M-H2O]- or [M + CH3COO]-, whereas APCI gives mainly the [M]- and [M - H]- ions. Quantification was carried out by flow injection. Calibration graphs were linear in the range of 10 ng to 1000 ng in TSP and 0.1 ng to 10 ng in APCI. Detection limits were in the range of 1 ng to 20 ng in TSP and 0.002 ng to 0.2 ng in APCI. The presence of BaP-1,6-dione, BaP-3,6-dione, and BaP-6,12-dione was confirmed in environmental samples of air particulate matter.
AB - The performance of two liquid chromatography-mass spectrometry (LC/MS) interfacing techniques, thermospray (TSP) and atmospheric pressure chemical ionization (APCI), for the analysis of benzo[a]pyrene (BaP) metabolites (hydroxy, epoxy and quinone derivatives) was compared. Interface and detection parameters such as source temperature, eluent composition or flow rate were optimized using negative ion mode. In TSP, the main ions are mostly [M]-, [M-H2O]- or [M + CH3COO]-, whereas APCI gives mainly the [M]- and [M - H]- ions. Quantification was carried out by flow injection. Calibration graphs were linear in the range of 10 ng to 1000 ng in TSP and 0.1 ng to 10 ng in APCI. Detection limits were in the range of 1 ng to 20 ng in TSP and 0.002 ng to 0.2 ng in APCI. The presence of BaP-1,6-dione, BaP-3,6-dione, and BaP-6,12-dione was confirmed in environmental samples of air particulate matter.
UR - http://www.scopus.com/inward/record.url?scp=0001691573&partnerID=8YFLogxK
U2 - 10.1007/s002160050571
DO - 10.1007/s002160050571
M3 - Article
AN - SCOPUS:0001691573
SN - 0937-0633
VL - 359
SP - 267
EP - 273
JO - Fresenius' Journal of Analytical Chemistry
JF - Fresenius' Journal of Analytical Chemistry
IS - 3
ER -