Comparison of a novel micro-assay for insulin autoantibodies with the conventional radiobinding assay

H. E. Naserke; N. Dozio; A. G. Ziegler; E. Bonifacio

doi:10.1007/s001250050968

Comparison of a novel micro-assay for insulin autoantibodies with the conventional radiobinding assay

H. E. Naserke, N. Dozio, A. G. Ziegler, E. Bonifacio

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

Measurement of insulin autoantibodies (IAA) with a novel micro radiobinding assay which requires only 20 μl of serum was compared with that in a conventional radiobinding assay which uses 600 μl of serum. IAA were measured with both assays in samples from 94 new onset insulin-dependent diabetes mellitus (IDDM) patients, 97 control subjects, and 48 first degree relatives of IDDM patients selected for having IAA in the conventional radiobinding assay. Overall, 227 (95%) of 239 samples tested were concordant, and IAA levels correlated well (r² = 0.7) between the two assays. Discordant results were obtained in 7 new onset patients, 4 control subjects, and 1 first degree relative, and these had low IAA levels in the respective assays. Sensitivity and specificity in the new onset IDDM patients and control subjects were 69% and 98% for the micro radiobinding assay and 72% and 98% for the conventional radiobinding assay. The use of the micro radiobinding assay should greatly facilitate islet related antibody screening, particularly in children.

Original language	English
Pages (from-to)	681-683
Number of pages	3
Journal	Diabetologia
Volume	41
Issue number	6
DOIs	https://doi.org/10.1007/s001250050968
State	Published - 1998
Externally published	Yes

Keywords

Insulin autoantibodies
Insulin-dependent diabetes mellitus
Prediction
Radiobinding assay

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10.1007/s001250050968

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title = "Comparison of a novel micro-assay for insulin autoantibodies with the conventional radiobinding assay",

abstract = "Measurement of insulin autoantibodies (IAA) with a novel micro radiobinding assay which requires only 20 μl of serum was compared with that in a conventional radiobinding assay which uses 600 μl of serum. IAA were measured with both assays in samples from 94 new onset insulin-dependent diabetes mellitus (IDDM) patients, 97 control subjects, and 48 first degree relatives of IDDM patients selected for having IAA in the conventional radiobinding assay. Overall, 227 (95%) of 239 samples tested were concordant, and IAA levels correlated well (r2 = 0.7) between the two assays. Discordant results were obtained in 7 new onset patients, 4 control subjects, and 1 first degree relative, and these had low IAA levels in the respective assays. Sensitivity and specificity in the new onset IDDM patients and control subjects were 69% and 98% for the micro radiobinding assay and 72% and 98% for the conventional radiobinding assay. The use of the micro radiobinding assay should greatly facilitate islet related antibody screening, particularly in children.",

keywords = "Insulin autoantibodies, Insulin-dependent diabetes mellitus, Prediction, Radiobinding assay",

author = "Naserke, {H. E.} and N. Dozio and Ziegler, {A. G.} and E. Bonifacio",

note = "Funding Information: Acknowledgements. We thank Annette Schimmel and Cristina Belloni for their help in sample preparation and antibody testing. This study forms part of the dissertation of H. E. Naserke at the Ludwig-Maximillians-University of Munich. It was supported by DLR grant FKZ:01KD9601/7and by the EU concerted action PARADIGM.",

year = "1998",

doi = "10.1007/s001250050968",

language = "English",

volume = "41",

pages = "681--683",

journal = "Diabetologia",

issn = "0012-186X",

publisher = "Springer Science and Business Media Deutschland GmbH",

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AU - Naserke, H. E.

AU - Dozio, N.

AU - Ziegler, A. G.

AU - Bonifacio, E.

N1 - Funding Information: Acknowledgements. We thank Annette Schimmel and Cristina Belloni for their help in sample preparation and antibody testing. This study forms part of the dissertation of H. E. Naserke at the Ludwig-Maximillians-University of Munich. It was supported by DLR grant FKZ:01KD9601/7and by the EU concerted action PARADIGM.

PY - 1998

Y1 - 1998

N2 - Measurement of insulin autoantibodies (IAA) with a novel micro radiobinding assay which requires only 20 μl of serum was compared with that in a conventional radiobinding assay which uses 600 μl of serum. IAA were measured with both assays in samples from 94 new onset insulin-dependent diabetes mellitus (IDDM) patients, 97 control subjects, and 48 first degree relatives of IDDM patients selected for having IAA in the conventional radiobinding assay. Overall, 227 (95%) of 239 samples tested were concordant, and IAA levels correlated well (r2 = 0.7) between the two assays. Discordant results were obtained in 7 new onset patients, 4 control subjects, and 1 first degree relative, and these had low IAA levels in the respective assays. Sensitivity and specificity in the new onset IDDM patients and control subjects were 69% and 98% for the micro radiobinding assay and 72% and 98% for the conventional radiobinding assay. The use of the micro radiobinding assay should greatly facilitate islet related antibody screening, particularly in children.

AB - Measurement of insulin autoantibodies (IAA) with a novel micro radiobinding assay which requires only 20 μl of serum was compared with that in a conventional radiobinding assay which uses 600 μl of serum. IAA were measured with both assays in samples from 94 new onset insulin-dependent diabetes mellitus (IDDM) patients, 97 control subjects, and 48 first degree relatives of IDDM patients selected for having IAA in the conventional radiobinding assay. Overall, 227 (95%) of 239 samples tested were concordant, and IAA levels correlated well (r2 = 0.7) between the two assays. Discordant results were obtained in 7 new onset patients, 4 control subjects, and 1 first degree relative, and these had low IAA levels in the respective assays. Sensitivity and specificity in the new onset IDDM patients and control subjects were 69% and 98% for the micro radiobinding assay and 72% and 98% for the conventional radiobinding assay. The use of the micro radiobinding assay should greatly facilitate islet related antibody screening, particularly in children.

KW - Insulin autoantibodies

KW - Insulin-dependent diabetes mellitus

KW - Prediction

KW - Radiobinding assay

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Comparison of a novel micro-assay for insulin autoantibodies with the conventional radiobinding assay

Abstract

Keywords

UN SDGs

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