TY - JOUR
T1 - Comparative gene array analysis of progenitor cells from human paired deep neck and subcutaneous adipose tissue
AU - Tews, D.
AU - Schwar, V.
AU - Scheithauer, M.
AU - Weber, T.
AU - Fromme, T.
AU - Klingenspor, M.
AU - Barth, T. F.
AU - Möller, P.
AU - Holzmann, K.
AU - Debatin, K. M.
AU - Fischer-Posovszky, P.
AU - Wabitsch, M.
N1 - Funding Information:
This work was supported by a grant from the Ministry of Science, Research and the Arts of Baden-Württemberg (Boehringer Ingelheim University Ulm Biocenter (BIU), Az: 32-7533.-6-10/15/5 ) to DT and by a grant of the German Federal Ministry for Education and Research (BMBF, project funding reference number (FKZ: 01GI1326 ) and is integrated in the Competence Network Obesity (CNO). MK was supported by the Else Kröner-Fresenius Foundation as well as grants from the German Federal Ministry for Education and Research (BMBF: NGFNplus FKZ 01GS0822 and CNO FKZ 01G|1325 ).
PY - 2014/9
Y1 - 2014/9
N2 - In this study we sought to clarify the molecular differences between human brown and white adipocyte progenitors cells. To this end, we performed comparative gene array analysis on progenitor cells isolated from paired biopsies of deep and subcutaneous neck adipose tissue from individuals (n=6) undergoing neck surgery. Compared with subcutaneous neck progenitors, cells from the deep neck adipose tissue displayed marked differences in gene expression pattern, including 355 differentially regulated (>1.5 fold) genes. Analysis of highest regulated genes revealed that STMN2, MME, ODZ2, NRN1 and IL13RA2 genes were specifically expressed in white progenitor cells, whereas expression of LRRC17, CNTNAP3, CD34, RGS7BP and ADH1B marked brown progenitor cells. In conclusion, progenitors from deep neck and subcutaneous neck adipose tissue are characterized by a distinct molecular signature, giving rise to either brown or white adipocytes. The newly identified markers may provide potential pharmacological targets facilitating brown adipogenesis.
AB - In this study we sought to clarify the molecular differences between human brown and white adipocyte progenitors cells. To this end, we performed comparative gene array analysis on progenitor cells isolated from paired biopsies of deep and subcutaneous neck adipose tissue from individuals (n=6) undergoing neck surgery. Compared with subcutaneous neck progenitors, cells from the deep neck adipose tissue displayed marked differences in gene expression pattern, including 355 differentially regulated (>1.5 fold) genes. Analysis of highest regulated genes revealed that STMN2, MME, ODZ2, NRN1 and IL13RA2 genes were specifically expressed in white progenitor cells, whereas expression of LRRC17, CNTNAP3, CD34, RGS7BP and ADH1B marked brown progenitor cells. In conclusion, progenitors from deep neck and subcutaneous neck adipose tissue are characterized by a distinct molecular signature, giving rise to either brown or white adipocytes. The newly identified markers may provide potential pharmacological targets facilitating brown adipogenesis.
KW - Adipogenesis
KW - Brown adipose tissue
KW - Obesity
KW - Progenitor cells
UR - http://www.scopus.com/inward/record.url?scp=84906519417&partnerID=8YFLogxK
U2 - 10.1016/j.mce.2014.07.011
DO - 10.1016/j.mce.2014.07.011
M3 - Article
C2 - 25102227
AN - SCOPUS:84906519417
SN - 0303-7207
VL - 395
SP - 41
EP - 50
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -
