TY - JOUR
T1 - Comparative evaluation of the angiogenic potential of hypoxia preconditioned blood-derived secretomes and platelet-rich plasma
T2 - An in vitro analysis
AU - Moog, Philipp
AU - Kirchhoff, Katharina
AU - Bekeran, Sanjar
AU - Bauer, Anna Theresa
AU - Isenburg, Sarah von
AU - Dornseifer, Ulf
AU - Machens, Hans Günther
AU - Schilling, Arndt F.
AU - Hadjipanayi, Ektoras
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Blood-derived factor preparations are being clinically employed as tools for promoting tissue repair and regeneration. Here we set out to characterize the in vitro angiogenic potential of two types of frequently used autologous blood-derived secretomes: platelet-rich plasma (PRP) and hypoxia preconditioned plasma (HPP)/serum (HPS). The concentration of key pro-angiogenic (VEGF) and anti-angiogenic (TSP-1, PF-4) protein factors in these secretomes was analyzed via ELISA, while their ability to induce microvessel formation and sprouting was examined in endothelial cell and aortic ring cultures, respectively. We found higher concentrations of VEGF in PRP and HPP/HPS compared to normal plasma and serum. This correlated with improved induction of microvessel formation by PRP and HPP/HPS. HPP had a significantly lower TSP-1 and PF-4 concentration than PRP and HPS. PRP and HPP/HPS appeared to induce similar levels of microvessel sprouting; however, the length of these sprouts was greater in HPP/HPS than in PRP cultures. A bell-shaped angiogenic response profile was observed with increasing HPP/HPS dilutions, with peak values significantly exceeding the PRP response. Our findings demonstrate that optimization of peripheral blood cell-derived angiogenic factor signalling through hypoxic preconditioning offers an improved alternative to simple platelet concentration and release of growth factors pre-stored in platelets.
AB - Blood-derived factor preparations are being clinically employed as tools for promoting tissue repair and regeneration. Here we set out to characterize the in vitro angiogenic potential of two types of frequently used autologous blood-derived secretomes: platelet-rich plasma (PRP) and hypoxia preconditioned plasma (HPP)/serum (HPS). The concentration of key pro-angiogenic (VEGF) and anti-angiogenic (TSP-1, PF-4) protein factors in these secretomes was analyzed via ELISA, while their ability to induce microvessel formation and sprouting was examined in endothelial cell and aortic ring cultures, respectively. We found higher concentrations of VEGF in PRP and HPP/HPS compared to normal plasma and serum. This correlated with improved induction of microvessel formation by PRP and HPP/HPS. HPP had a significantly lower TSP-1 and PF-4 concentration than PRP and HPS. PRP and HPP/HPS appeared to induce similar levels of microvessel sprouting; however, the length of these sprouts was greater in HPP/HPS than in PRP cultures. A bell-shaped angiogenic response profile was observed with increasing HPP/HPS dilutions, with peak values significantly exceeding the PRP response. Our findings demonstrate that optimization of peripheral blood cell-derived angiogenic factor signalling through hypoxic preconditioning offers an improved alternative to simple platelet concentration and release of growth factors pre-stored in platelets.
KW - Angiogenesis
KW - Blood-derived therapy
KW - Hypoxia
KW - Hypoxia preconditioned plasma
KW - Hypoxia preconditioned serum
KW - Peripheral blood cells
KW - Platelet rich plasma (PRP)
UR - http://www.scopus.com/inward/record.url?scp=85078851216&partnerID=8YFLogxK
U2 - 10.3390/biomedicines8010016
DO - 10.3390/biomedicines8010016
M3 - Article
AN - SCOPUS:85078851216
SN - 2227-9059
VL - 8
JO - Biomedicines
JF - Biomedicines
IS - 1
M1 - 16
ER -