Comparative analysis of core proteomes of human cell lines by 1D PAGE and LC-MS/MS

Markus E. Schirle; Manfred Raida; Yann Abraham; Gerlinde Stark; Gitte Neubauer; Bernhard Kuster

Comparative analysis of core proteomes of human cell lines by 1D PAGE and LC-MS/MS

Markus E. Schirle
, Manfred Raida
, Yann Abraham
, Gerlinde Stark
, Gitte Neubauer
, Bernhard Kuster

Cellzome GmbH

Research output: Contribution to conference › Paper › peer-review

Abstract

The cytoplasmic core proteomes of three human cell lines were analyzed by nano LC-MS/MS: HEK293 (embryonic kidney cells), SKNBE2(neuroblastoma) and Hep G2 (heptacellular carcinoma). Cell lysates were centrifuged to obtain a post nuclear supernatant which was further separated into a cytoplasmic fraction and a membrane fraction. The influence of subcellular fractionation on protein identification, the membrane fraction and total cell lysate of HEK293 cells were also analyzed. It was found that the percentage of proteins identified in more than one subcellular fraction was higher compared to cytoplasmic fractions from different cell lines.

Original language	English
Pages	267-268
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/06/02 → 6/06/02

Cite this

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title = "Comparative analysis of core proteomes of human cell lines by 1D PAGE and LC-MS/MS",

abstract = "The cytoplasmic core proteomes of three human cell lines were analyzed by nano LC-MS/MS: HEK293 (embryonic kidney cells), SKNBE2(neuroblastoma) and Hep G2 (heptacellular carcinoma). Cell lysates were centrifuged to obtain a post nuclear supernatant which was further separated into a cytoplasmic fraction and a membrane fraction. The influence of subcellular fractionation on protein identification, the membrane fraction and total cell lysate of HEK293 cells were also analyzed. It was found that the percentage of proteins identified in more than one subcellular fraction was higher compared to cytoplasmic fractions from different cell lines.",

author = "Schirle, \{Markus E.\} and Manfred Raida and Yann Abraham and Gerlinde Stark and Gitte Neubauer and Bernhard Kuster",

year = "2002",

language = "English",

pages = "267--268",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - CONF

T1 - Comparative analysis of core proteomes of human cell lines by 1D PAGE and LC-MS/MS

AU - Schirle, Markus E.

AU - Raida, Manfred

AU - Abraham, Yann

AU - Stark, Gerlinde

AU - Neubauer, Gitte

AU - Kuster, Bernhard

PY - 2002

Y1 - 2002

N2 - The cytoplasmic core proteomes of three human cell lines were analyzed by nano LC-MS/MS: HEK293 (embryonic kidney cells), SKNBE2(neuroblastoma) and Hep G2 (heptacellular carcinoma). Cell lysates were centrifuged to obtain a post nuclear supernatant which was further separated into a cytoplasmic fraction and a membrane fraction. The influence of subcellular fractionation on protein identification, the membrane fraction and total cell lysate of HEK293 cells were also analyzed. It was found that the percentage of proteins identified in more than one subcellular fraction was higher compared to cytoplasmic fractions from different cell lines.

AB - The cytoplasmic core proteomes of three human cell lines were analyzed by nano LC-MS/MS: HEK293 (embryonic kidney cells), SKNBE2(neuroblastoma) and Hep G2 (heptacellular carcinoma). Cell lysates were centrifuged to obtain a post nuclear supernatant which was further separated into a cytoplasmic fraction and a membrane fraction. The influence of subcellular fractionation on protein identification, the membrane fraction and total cell lysate of HEK293 cells were also analyzed. It was found that the percentage of proteins identified in more than one subcellular fraction was higher compared to cytoplasmic fractions from different cell lines.

UR - https://www.scopus.com/pages/publications/2442674326

M3 - Paper

AN - SCOPUS:2442674326

SP - 267

EP - 268

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Comparative analysis of core proteomes of human cell lines by 1D PAGE and LC-MS/MS

Abstract

Conference

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