Abstract
The cytoplasmic core proteomes of three human cell lines were analyzed by nano LC-MS/MS: HEK293 (embryonic kidney cells), SKNBE2(neuroblastoma) and Hep G2 (heptacellular carcinoma). Cell lysates were centrifuged to obtain a post nuclear supernatant which was further separated into a cytoplasmic fraction and a membrane fraction. The influence of subcellular fractionation on protein identification, the membrane fraction and total cell lysate of HEK293 cells were also analyzed. It was found that the percentage of proteins identified in more than one subcellular fraction was higher compared to cytoplasmic fractions from different cell lines.
| Original language | English |
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| Pages | 267-268 |
| Number of pages | 2 |
| State | Published - 2002 |
| Externally published | Yes |
| Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002 |
Conference
| Conference | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Country/Territory | United States |
| City | Orlando, FL |
| Period | 2/06/02 → 6/06/02 |