Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions

Dominik Soliman; George J. Tserevelakis; Murad Omar; Vasilis Ntziachristos

doi:10.1117/12.2208861

Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions

Dominik Soliman, George J. Tserevelakis, Murad Omar, Vasilis Ntziachristos

Chair of Biological Imaging

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Scopus citations

Abstract

We present a multi-scale imaging system that integrates five optoacoustic and multi-photon modalities into the same device. The hybrid microscope offers a unique zoom-in ability by allowing for optoacoustic microscopy and mesoscopy scans of the sample within the same imaging framework. Furthermore, by combining several label-free modalities, we are able to visualize a broad range of anatomical features, taking advantage of their complementary contrast mechanisms. We characterize the spatial resolution and relative orientation of the different sub-modalities and demonstrate the system's performance by the imaging of several model organisms ex vivo. The presented ability to dynamically vary scanning volume and resolution together with its multi-contrast and label-free imaging capabilities make the hybrid microscope a promising tool for comprehensive biological imaging.

Original language	English
Title of host publication	Photons Plus Ultrasound
Subtitle of host publication	Imaging and Sensing 2016
Editors	Alexander A. Oraevsky, Lihong V. Wang
Publisher	SPIE
ISBN (Electronic)	9781628419429
DOIs	https://doi.org/10.1117/12.2208861
State	Published - 2016
Event	Photons Plus Ultrasound: Imaging and Sensing 2016 - San Francisco, United States Duration: 14 Feb 2016 → 17 Feb 2016

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9708
ISSN (Print)	1605-7422

Conference

Conference	Photons Plus Ultrasound: Imaging and Sensing 2016
Country/Territory	United States
City	San Francisco
Period	14/02/16 → 17/02/16

Keywords

Mesoscopy
Multi-modal imaging
Multi-scale imaging
Multiphoton microscopy
Optoacoustic microscopy
Photoacoustic microscopy

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10.1117/12.2208861

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Soliman, D., Tserevelakis, G. J., Omar, M., & Ntziachristos, V. (2016). Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions. In A. A. Oraevsky, & L. V. Wang (Eds.), Photons Plus Ultrasound: Imaging and Sensing 2016 Article 97083B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9708). SPIE. https://doi.org/10.1117/12.2208861

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abstract = "We present a multi-scale imaging system that integrates five optoacoustic and multi-photon modalities into the same device. The hybrid microscope offers a unique zoom-in ability by allowing for optoacoustic microscopy and mesoscopy scans of the sample within the same imaging framework. Furthermore, by combining several label-free modalities, we are able to visualize a broad range of anatomical features, taking advantage of their complementary contrast mechanisms. We characterize the spatial resolution and relative orientation of the different sub-modalities and demonstrate the system's performance by the imaging of several model organisms ex vivo. The presented ability to dynamically vary scanning volume and resolution together with its multi-contrast and label-free imaging capabilities make the hybrid microscope a promising tool for comprehensive biological imaging.",

keywords = "Mesoscopy, Multi-modal imaging, Multi-scale imaging, Multiphoton microscopy, Optoacoustic microscopy, Photoacoustic microscopy",

author = "Dominik Soliman and Tserevelakis, {George J.} and Murad Omar and Vasilis Ntziachristos",

note = "Publisher Copyright: {\textcopyright} 2016 SPIE.; Photons Plus Ultrasound: Imaging and Sensing 2016 ; Conference date: 14-02-2016 Through 17-02-2016",

year = "2016",

doi = "10.1117/12.2208861",

language = "English",

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Soliman, D, Tserevelakis, GJ, Omar, M & Ntziachristos, V 2016, Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions. in AA Oraevsky & LV Wang (eds), Photons Plus Ultrasound: Imaging and Sensing 2016., 97083B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9708, SPIE, Photons Plus Ultrasound: Imaging and Sensing 2016, San Francisco, United States, 14/02/16. https://doi.org/10.1117/12.2208861

Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions. / Soliman, Dominik; Tserevelakis, George J.; Omar, Murad et al.
Photons Plus Ultrasound: Imaging and Sensing 2016. ed. / Alexander A. Oraevsky; Lihong V. Wang. SPIE, 2016. 97083B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9708).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Tserevelakis, George J.

AU - Omar, Murad

AU - Ntziachristos, Vasilis

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N2 - We present a multi-scale imaging system that integrates five optoacoustic and multi-photon modalities into the same device. The hybrid microscope offers a unique zoom-in ability by allowing for optoacoustic microscopy and mesoscopy scans of the sample within the same imaging framework. Furthermore, by combining several label-free modalities, we are able to visualize a broad range of anatomical features, taking advantage of their complementary contrast mechanisms. We characterize the spatial resolution and relative orientation of the different sub-modalities and demonstrate the system's performance by the imaging of several model organisms ex vivo. The presented ability to dynamically vary scanning volume and resolution together with its multi-contrast and label-free imaging capabilities make the hybrid microscope a promising tool for comprehensive biological imaging.

AB - We present a multi-scale imaging system that integrates five optoacoustic and multi-photon modalities into the same device. The hybrid microscope offers a unique zoom-in ability by allowing for optoacoustic microscopy and mesoscopy scans of the sample within the same imaging framework. Furthermore, by combining several label-free modalities, we are able to visualize a broad range of anatomical features, taking advantage of their complementary contrast mechanisms. We characterize the spatial resolution and relative orientation of the different sub-modalities and demonstrate the system's performance by the imaging of several model organisms ex vivo. The presented ability to dynamically vary scanning volume and resolution together with its multi-contrast and label-free imaging capabilities make the hybrid microscope a promising tool for comprehensive biological imaging.

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KW - Multi-modal imaging

KW - Multi-scale imaging

KW - Multiphoton microscopy

KW - Optoacoustic microscopy

KW - Photoacoustic microscopy

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BT - Photons Plus Ultrasound

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ER -

Combined label-free optical and optoacoustic imaging of model organisms at mesoscopy and microscopy resolutions

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Keywords

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