cmRNA/lipoplex encapsulation in PLGA microspheres enables transfection via calcium phosphate cement (CPC)/PLGA composites

Maximilian Utzinger, Anita Jarzebinska, Nicolas Haag, Martin Schweizer, Gerhard Winter, Christian Dohmen, Carsten Rudolph, Christian Plank

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

In this study lipoplexes containing chemically modified messenger RNA (cmRNA) were incorporated into poly (lactic-co-glycolic acid) (PLGA) microspheres via water-in-oil-in-water (W/O/W) double emulsion solvent evaporation technique. The nanoparticle encapsulation by microparticle formation was optimized to achieve lipoplex release and maximum transfection efficiency in surrounding cells. It was possible to adjust characteristic features in surface topology and size of the PLGA-microspheres by varying the extent of lipoplex loading into the polymer matrix. The partial release of lipids and mRNA out of the microparticle system, their accumulation in cells and the production of encoded protein were visualized via fluorescence microscopy. These bioactive microspheres, containing cmRNA bearing lipoplexes, were developed for the incorporation of a therapeutic component into injectable calcium phosphate cements (CPC). Due to the incorporation of PLGA/lipoplex microspheres as a degradable entity, the porosity of the cement phase could additionally be adjusted. This approach of complex nanoparticle incorporation into polymer/cement composites represents a promising example for combining transcript therapy with biomechanical engineering.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalJournal of Controlled Release
Volume249
DOIs
StatePublished - 10 Mar 2017

Keywords

  • Bone substitute materials
  • Calcium phosphate cements
  • Lipoplexes
  • Microparticles
  • PLGA
  • Transcript therapy

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